Isotropic 3D Super-resolution Imaging with a Self-bending Point Spread Function
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CitationJia, Shu, Joshua C. Vaughan, and Xiaowei Zhuang. 2014. “Isotropic 3D Super-resolution Imaging with a Self-bending Point Spread Function.” Nature photonics 8 (1): 302-306. doi:10.1038/nphoton.2014.13. http://dx.doi.org/10.1038/nphoton.2014.13.
AbstractAiry beams maintain their intensity profiles over a large propagation distance without substantial diffraction and exhibit lateral bending during propagation1-5. This unique property has been exploited for micromanipulation of particles6, generation of plasma channels7 and guidance of plasmonic waves8, but has not been explored for high-resolution optical microscopy. Here, we introduce a self-bending point spread function (SB-PSF) based on Airy beams for three-dimensional (3D) super-resolution fluorescence imaging. We designed a side-lobe-free SB-PSF and implemented a two-channel detection scheme to enable unambiguous 3D localization of fluorescent molecules. The lack of diffraction and the propagation-dependent lateral bending make the SB-PSF well suited for precise 3D localization of molecules over a large imaging depth. Using this method, we obtained super-resolution imaging with isotropic 3D localization precision of 10-15 nm over a 3 μm imaging depth from ∼2000 photons per localization.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13454694
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