dc.contributor.author | Lohr, Jens G. | en_US |
dc.contributor.author | Adalsteinsson, Viktor A. | en_US |
dc.contributor.author | Cibulskis, Kristian | en_US |
dc.contributor.author | Choudhury, Atish D. | en_US |
dc.contributor.author | Rosenberg, Mara | en_US |
dc.contributor.author | Cruz-Gordillo, Peter | en_US |
dc.contributor.author | Francis, Joshua | en_US |
dc.contributor.author | Zhang, Cheng-Zhong | en_US |
dc.contributor.author | Shalek, Alex K. | en_US |
dc.contributor.author | Satija, Rahul | en_US |
dc.contributor.author | Trombetta, John T. | en_US |
dc.contributor.author | Lu, Diana | en_US |
dc.contributor.author | Tallapragada, Naren | en_US |
dc.contributor.author | Tahirova, Narmin | en_US |
dc.contributor.author | Kim, Sora | en_US |
dc.contributor.author | Blumenstiel, Brendan | en_US |
dc.contributor.author | Sougnez, Carrie | en_US |
dc.contributor.author | Lowe, Alarice | en_US |
dc.contributor.author | Wong, Bang | en_US |
dc.contributor.author | Auclair, Daniel | en_US |
dc.contributor.author | Van Allen, Eliezer M. | en_US |
dc.contributor.author | Nakabayashi, Mari | en_US |
dc.contributor.author | Lis, Rosina T. | en_US |
dc.contributor.author | Lee, Gwo-Shu M. | en_US |
dc.contributor.author | Li, Tiantian | en_US |
dc.contributor.author | Chabot, Matthew S. | en_US |
dc.contributor.author | Ly, Amy | en_US |
dc.contributor.author | Taplin, Mary-Ellen | en_US |
dc.contributor.author | Clancy, Thomas E. | en_US |
dc.contributor.author | Loda, Massimo | en_US |
dc.contributor.author | Regev, Aviv | en_US |
dc.contributor.author | Meyerson, Matthew | en_US |
dc.contributor.author | Hahn, William C. | en_US |
dc.contributor.author | Kantoff, Philip W. | en_US |
dc.contributor.author | Golub, Todd R. | en_US |
dc.contributor.author | Getz, Gad | en_US |
dc.contributor.author | Boehm, Jesse S. | en_US |
dc.contributor.author | Love, J. Christopher | en_US |
dc.date.accessioned | 2014-12-02T21:28:36Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Lohr, J. G., V. A. Adalsteinsson, K. Cibulskis, A. D. Choudhury, M. Rosenberg, P. Cruz-Gordillo, J. Francis, et al. 2014. “Whole exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer.” Nature biotechnology 32 (5): 479-484. doi:10.1038/nbt.2892. http://dx.doi.org/10.1038/nbt.2892. | en |
dc.identifier.issn | 1087-0156 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:13454751 | |
dc.description.abstract | Comprehensive analyses of cancer genomes promise to inform prognoses and precise cancer treatments. A major barrier, however, is inaccessibility of metastatic tissue. A potential solution is to characterize circulating tumor cells (CTCs), but this requires overcoming the challenges of isolating rare cells and sequencing low-input material. Here we report an integrated process to isolate, qualify and sequence whole exomes of CTCs with high fidelity, using a census-based sequencing strategy. Power calculations suggest that mapping of >99.995% of the standard exome is possible in CTCs. We validated our process in two prostate cancer patients including one for whom we sequenced CTCs, a lymph node metastasis and nine cores of the primary tumor. Fifty-one of 73 CTC mutations (70%) were observed in matched tissue. Moreover, we identified 10 early-trunk and 56 metastatic-trunk mutations in the non-CTC tumor samples and found 90% and 73% of these, respectively, in CTC exomes. This study establishes a foundation for CTC genomics in the clinic. | en |
dc.language.iso | en_US | en |
dc.relation.isversionof | doi:10.1038/nbt.2892 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4034575/pdf/ | en |
dash.license | LAA | en_US |
dc.title | Whole exome sequencing of circulating tumor cells provides a window into metastatic prostate cancer | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | Nature biotechnology | en |
dash.depositing.author | Lohr, Jens G. | en_US |
dc.date.available | 2014-12-02T21:28:36Z | |
dc.identifier.doi | 10.1038/nbt.2892 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Choudhury, Atish | |
dash.contributor.affiliated | Lowe, Alarice | |
dash.contributor.affiliated | Nakabayashi, Mari | |
dash.contributor.affiliated | Clancy, Thomas | |
dash.contributor.affiliated | Kantoff, Philip | |
dash.contributor.affiliated | Taplin, Mary-Ellen | |
dash.contributor.affiliated | Ly, Amy | |
dash.contributor.affiliated | Golub, Todd | |
dash.contributor.affiliated | Lohr, Jens | |
dash.contributor.affiliated | Meyerson, Matthew | |
dash.contributor.affiliated | Hahn, William | |
dash.contributor.affiliated | Loda, Massimo | |
dash.contributor.affiliated | Van Allen, Eliezer | |
dash.contributor.affiliated | Getz, Gad | |