The Genome of the Chicken DT40 Bursal Lymphoma Cell Line
View/ Open
Author
Molnár, János
Póti, Ádám
Pipek, Orsolya
Krzystanek, Marcin
Kanu, Nnennaya
Swanton, Charles
Tusnády, Gábor E.
Csabai, István
Szüts, Dávid
Published Version
https://doi.org/10.1534/g3.114.013482Metadata
Show full item recordCitation
Molnár, János, Ádám Póti, Orsolya Pipek, Marcin Krzystanek, Nnennaya Kanu, Charles Swanton, Gábor E. Tusnády, Zoltan Szallasi, István Csabai, and Dávid Szüts. 2014. “The Genome of the Chicken DT40 Bursal Lymphoma Cell Line.” G3: Genes|Genomes|Genetics 4 (11): 2231-2240. doi:10.1534/g3.114.013482. http://dx.doi.org/10.1534/g3.114.013482.Abstract
The chicken DT40 cell line is a widely used model system in the study of multiple cellular processes due to the efficiency of homologous gene targeting. The cell line was derived from a bursal lymphoma induced by avian leukosis virus infection. In this study we characterized the genome of the cell line using whole genome shotgun sequencing and single nucleotide polymorphism array hybridization. The results indicate that wild-type DT40 has a relatively normal karyotype, except for whole chromosome copy number gains, and no karyotype variability within stocks. In a comparison to two domestic chicken genomes and the Gallus gallus reference genome, we found no unique mutational processes shaping the DT40 genome except for a mild increase in insertion and deletion events, particularly deletions at tandem repeats. We mapped coding sequence mutations that are unique to the DT40 genome; mutations inactivating the PIK3R1 and ATRX genes likely contributed to the oncogenic transformation. In addition to a known avian leukosis virus integration in the MYC gene, we detected further integration sites that are likely to de-regulate gene expression. The new findings support the hypothesis that DT40 is a typical transformed cell line with a relatively intact genome; therefore, it is well-suited to the role of a model system for DNA repair and related processes. The sequence data generated by this study, including a searchable de novo genome assembly and annotated lists of mutated genes, will support future research using this cell line.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4232548/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:13454762
Collections
- HMS Scholarly Articles [17922]
Contact administrator regarding this item (to report mistakes or request changes)