Scalable Generation of Universal Platelets from Human Induced Pluripotent Stem Cells
Thon, Jonathan N.
Machlus, Kellie R.
Kimbrel, Erin A.
Lanza, RobertNote: Order does not necessarily reflect citation order of authors.
MetadataShow full item record
CitationFeng, Q., N. Shabrani, J. Thon, H. Huo, A. Thiel, K. Machlus, K. Kim, et al. 2014. “Scalable Generation of Universal Platelets from Human Induced Pluripotent Stem Cells.” Stem Cell Reports 3 (5): 817-831. doi:10.1016/j.stemcr.2014.09.010. http://dx.doi.org/10.1016/j.stemcr.2014.09.010.
AbstractSummary Human induced pluripotent stem cells (iPSCs) provide a potentially replenishable source for the production of transfusable platelets. Here, we describe a method to generate megakaryocytes (MKs) and functional platelets from iPSCs in a scalable manner under serum/feeder-free conditions. The method also permits the cryopreservation of MK progenitors, enabling a rapid “surge” capacity when large numbers of platelets are needed. Ultrastructural/morphological analyses show no major differences between iPSC platelets and human blood platelets. iPSC platelets form aggregates, lamellipodia, and filopodia after activation and circulate in macrophage-depleted animals and incorporate into developing mouse thrombi in a manner identical to human platelets. By knocking out the β2-microglobulin gene, we have generated platelets that are negative for the major histocompatibility antigens. The scalable generation of HLA-ABC-negative platelets from a renewable cell source represents an important step toward generating universal platelets for transfusion as well as a potential strategy for the management of platelet refractoriness.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13454781
- HMS Scholarly Articles