Flt3L-dependence helps define an uncharacterized subset of murine cutaneous dendritic cells

DSpace/Manakin Repository

Flt3L-dependence helps define an uncharacterized subset of murine cutaneous dendritic cells

Citable link to this page

 

 
Title: Flt3L-dependence helps define an uncharacterized subset of murine cutaneous dendritic cells
Author: Mollah, Shamim; Dobrin, Joseph; Feder, Rachel; Tse, Sze-Wah; Matos, Ines; Cheong, Cheolho; Steinman, Ralph M.; Anandasabapathy, Niroshana

Note: Order does not necessarily reflect citation order of authors.

Citation: Mollah, Shamim, Joseph Dobrin, Rachel Feder, Sze-Wah Tse, Ines Matos, Cheolho Cheong, Ralph M. Steinman, and Niroshana Anandasabapathy. 2014. “Flt3L-dependence helps define an uncharacterized subset of murine cutaneous dendritic cells.” The Journal of investigative dermatology 134 (5): 1265-1275. doi:10.1038/jid.2013.515. http://dx.doi.org/10.1038/jid.2013.515.
Full Text & Related Files:
Abstract: Skin-derived dendritic cells (DC) are potent antigen presenting cells with critical roles in both adaptive immunity and tolerance to self. Skin DC carry antigens and constitutively migrate to the skin draining lymph nodes (LN). In mice, Langerin-CD11b− dermal DC are a low-frequency, heterogeneous, migratory DC subset that traffic to LN (Langerin-CD11b-migDC). Here, we build on the observation that Langerin-CD11b− migDC are Fms-like tyrosine kinase 3 ligand (Flt3L) dependent and strongly Flt3L responsive, which may relate them to classical DCs. Examination of DC capture of FITC from painted skin, DC isolation from skin explant culture, and from the skin of CCR7 knockout mice which accumulate migDC, demonstrate these cells are cutaneous residents. Langerin-CD11b-Flt3L responsive DC are largely CD24(+) and CX3CR1low and can be depleted from Zbtb46-DTR mice, suggesting classical DC lineage. Langerin-CD11bmigDC present antigen with equal efficiency to other DC subsets ex vivo including classical CD8α cDC and Langerin+CD103+ dermal DC. Finally, transcriptome analysis suggests a close relationship to other skin DC, and a lineage relationship to other classical DC. This work demonstrates that Langerin- CD11b− dermal DC, a previously overlooked cell subset, may be an important player in the cutaneous immune environment.
Published Version: doi:10.1038/jid.2013.515
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994898/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:13454847
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters