Show simple item record

dc.contributor.authorLu, Lei
dc.contributor.authorHannoush, Rami N.
dc.contributor.authorGoess, Brian C.
dc.contributor.authorVaradarajan, Shankar
dc.contributor.authorShair, Matthew David
dc.contributor.authorKirchhausen, Tomas Leopold
dc.date.accessioned2014-12-03T18:43:14Z
dash.embargo.terms2014-05-01
dc.date.issued2013
dc.identifier.citationLu, Lei, Rami N. Hannoush, Brian C. Goess, Shankar Varadarajan, Matthew David Shair, and Tomas Leopold Kirchhausen. 2013. "The Small Molecule Dispergo Tubulates the Endoplasmic Reticulum and Inhibits Export." Molecular Biology of the Cell 24 (7): 1020–1029.en_US
dc.identifier.issn1059-1524en_US
dc.identifier.issn1939-4586en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13457867
dc.description.abstractThe mammalian endoplasmic reticulum (ER) is an organelle that maintains a complex, compartmentalized organization of interconnected cisternae and tubules while supporting a continuous flow of newly synthesized proteins and lipids to the Golgi apparatus. Using a phenotypic screen, we identify a small molecule, dispergo, that induces reversible loss of the ER cisternae and extensive ER tubulation, including formation of ER patches comprising densely packed tubules. Dispergo also prevents export from the ER to the Golgi apparatus, and this traffic block results in breakdown of the Golgi apparatus, primarily due to maintenance of the constitutive retrograde transport of its components to the ER. The effects of dispergo are reversible, since its removal allows recovery of the ER cisternae at the expense of the densely packed tubular ER patches. This recovery occurs together with reactivation of ER-to-Golgi traffic and regeneration of a functional Golgi with correct morphology. Because dispergo is the first small molecule that reversibly tubulates the ER and inhibits its export function, it will be useful in studying these complex processes.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Cell Biologyen_US
dc.relation.isversionofdoi:10.1091/mbc.E12-08-0575en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3608490/pdf/1020.pdfen_US
dash.licenseLAA
dc.titleThe Small Molecule Dispergo Tubulates the Endoplasmic Reticulum and Inhibits Exporten_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalMolecular Biology of the Cellen_US
dash.depositing.authorShair, Matthew David
dc.date.available2014-12-04T08:30:55Z
dash.affiliation.otherHarvard Medical Schoolen_US
dc.identifier.doi10.1091/mbc.E12-08-0575*
workflow.legacycommentsFAR 2014. File obtained from NIH. Can deposit publisher's version after a 2-month embargo per publisher's licensing terms (see http://www.molbiolcell.org/site/misc/ifora.xhtml)en_US
dash.contributor.affiliatedLu, Lei
dash.contributor.affiliatedKirchhausen, Tomas
dash.contributor.affiliatedShair, Matthew


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record