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dc.contributor.authorSoh, Boon-Sengen_US
dc.contributor.authorBuac, Kristinaen_US
dc.contributor.authorXu, Huanshengen_US
dc.contributor.authorLi, Edwarden_US
dc.contributor.authorNg, Shi-Yanen_US
dc.contributor.authorWu, Haoen_US
dc.contributor.authorChmielowiec, Jolantaen_US
dc.contributor.authorJiang, Xinen_US
dc.contributor.authorBu, Leien_US
dc.contributor.authorLi, Ronald Aen_US
dc.contributor.authorCowan, Chaden_US
dc.contributor.authorChien, Kenneth Ren_US
dc.date.accessioned2015-01-05T18:25:54Z
dc.date.issued2014en_US
dc.identifier.citationSoh, B., K. Buac, H. Xu, E. Li, S. Ng, H. Wu, J. Chmielowiec, et al. 2014. “N-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironment.” Cell Research 24 (12): 1420-1432. doi:10.1038/cr.2014.142. http://dx.doi.org/10.1038/cr.2014.142.en
dc.identifier.issn1001-0602en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13581011
dc.description.abstractThe cardiac progenitor cells (CPCs) in the anterior heart field (AHF) are located in the pharyngeal mesoderm (PM), where they expand, migrate and eventually differentiate into major cell types found in the heart, including cardiomyocytes. The mechanisms by which these progenitors are able to expand within the PM microenvironment without premature differentiation remain largely unknown. Through in silico data mining, genetic loss-of-function studies, and in vivo genetic rescue studies, we identified N-cadherin and interaction with canonical Wnt signals as a critical component of the microenvironment that facilitates the expansion of AHF-CPCs in the PM. CPCs in N-cadherin mutant embryos were observed to be less proliferative and undergo premature differentiation in the PM. Notably, the phenotype of N-cadherin deficiency could be partially rescued by activating Wnt signaling, suggesting a delicate functional interaction between the adhesion role of N-cadherin and Wnt signaling in the early PM microenvironment. This study suggests a new mechanism for the early renewal of AHF progenitors where N-cadherin provides additional adhesion for progenitor cells in the PM, thereby allowing Wnt paracrine signals to expand the cells without premature differentiation.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/cr.2014.142en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4260345/pdf/en
dash.licenseLAAen_US
dc.subjectN-cadherinen
dc.subjectanterior heart fielden
dc.subjectcardiac progenitor cellsen
dc.subjectpremature differentiationen
dc.subjectWnt signalingen
dc.subjectmicroenvironmenten
dc.titleN-cadherin prevents the premature differentiation of anterior heart field progenitors in the pharyngeal mesodermal microenvironmenten
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalCell Researchen
dash.depositing.authorLi, Edwarden_US
dc.date.available2015-01-05T18:25:54Z
dc.identifier.doi10.1038/cr.2014.142*
dash.authorsorderedfalse
dash.contributor.affiliatedLi, Edward
dash.contributor.affiliatedJiang, Xin
dash.contributor.affiliatedCowan, Chad
dash.contributor.affiliatedWu, Hao


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