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dc.contributor.authorAlonso, Almaris N.en_US
dc.contributor.authorPerry, Kyle J.en_US
dc.contributor.authorRegeimbal, James M.en_US
dc.contributor.authorRegan, Patrick M.en_US
dc.contributor.authorHiggins, Darren E.en_US
dc.date.accessioned2015-01-05T18:25:58Z
dc.date.issued2014en_US
dc.identifier.citationAlonso, Almaris N., Kyle J. Perry, James M. Regeimbal, Patrick M. Regan, and Darren E. Higgins. 2014. “Identification of Listeria monocytogenes Determinants Required for Biofilm Formation.” PLoS ONE 9 (12): e113696. doi:10.1371/journal.pone.0113696. http://dx.doi.org/10.1371/journal.pone.0113696.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13581016
dc.description.abstractListeria monocytogenes is a Gram-positive, food-borne pathogen of humans and animals. L. monocytogenes is considered to be a potential public health risk by the U.S. Food and Drug Administration (FDA), as this bacterium can easily contaminate ready-to-eat (RTE) foods and cause an invasive, life-threatening disease (listeriosis). Bacteria can adhere and grow on multiple surfaces and persist within biofilms in food processing plants, providing resistance to sanitizers and other antimicrobial agents. While whole genome sequencing has led to the identification of biofilm synthesis gene clusters in many bacterial species, bioinformatics has not identified the biofilm synthesis genes within the L. monocytogenes genome. To identify genes necessary for L. monocytogenes biofilm formation, we performed a transposon mutagenesis library screen using a recently constructed Himar1 mariner transposon. Approximately 10,000 transposon mutants within L. monocytogenes strain 10403S were screened for biofilm formation in 96-well polyvinyl chloride (PVC) microtiter plates with 70 Himar1 insertion mutants identified that produced significantly less biofilms. DNA sequencing of the transposon insertion sites within the isolated mutants revealed transposon insertions within 38 distinct genetic loci. The identification of mutants bearing insertions within several flagellar motility genes previously known to be required for the initial stages of biofilm formation validated the ability of the mutagenesis screen to identify L. monocytogenes biofilm-defective mutants. Two newly identified genetic loci, dltABCD and phoPR, were selected for deletion analysis and both ΔdltABCD and ΔphoPR bacterial strains displayed biofilm formation defects in the PVC microtiter plate assay, confirming these loci contribute to biofilm formation by L. monocytogenes.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0113696en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269431/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and Life Sciencesen
dc.subjectBiochemistryen
dc.subjectProteinsen
dc.subjectEcologyen
dc.subjectMicrobial Ecologyen
dc.subjectBiofilmsen
dc.subjectBacterial Biofilmsen
dc.subjectGeneticsen
dc.subjectGenomicsen
dc.subjectMicrobial Genomicsen
dc.subjectBacterial Genomicsen
dc.subjectMolecular Geneticsen
dc.subjectMicrobiologyen
dc.subjectBacteriologyen
dc.subjectGram Positive Bacteriaen
dc.subjectMedical Microbiologyen
dc.subjectMicrobial Pathogensen
dc.subjectBacterial Pathogensen
dc.subjectMolecular Biologyen
dc.subjectMolecular Biology Techniquesen
dc.subjectMutagenesis and Gene Deletion Techniquesen
dc.subjectTransposon Mutagenesisen
dc.titleIdentification of Listeria monocytogenes Determinants Required for Biofilm Formationen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorAlonso, Almaris N.en_US
dc.date.available2015-01-05T18:25:58Z
dc.identifier.doi10.1371/journal.pone.0113696*
dash.contributor.affiliatedHiggins, Darren
dash.contributor.affiliatedRegeimbal, James M.
dash.contributor.affiliatedPerry, Kyle James
dash.contributor.affiliatedAlonso, Almaris N.


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