Show simple item record

dc.contributor.authorJin, Xiaotingen_US
dc.contributor.authorLi, Zhuoyuen_US
dc.contributor.authorSong, Lien_US
dc.contributor.authorLiu, Xiangyuanen_US
dc.contributor.authorChen, Meilanen_US
dc.contributor.authorCheng, Longen_US
dc.contributor.authorRen, Huaen_US
dc.date.accessioned2015-01-05T18:27:35Z
dc.date.issued2014en_US
dc.identifier.citationJin, Xiaoting, Li Song, Xiangyuan Liu, Meilan Chen, Zhuoyu Li, Long Cheng, and Hua Ren. 2014. “Protective Efficacy of Vitamins C and E on p,p′-DDT-Induced Cytotoxicity via the ROS-Mediated Mitochondrial Pathway and NF-κB/FasL Pathway.” PLoS ONE 9 (12): e113257. doi:10.1371/journal.pone.0113257. http://dx.doi.org/10.1371/journal.pone.0113257.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13581136
dc.description.abstractDichlorodiphenoxytrichloroethane (DDT) is a known persistent organic pollutant and liver damage toxicant. However, there has been little emphasis on the mechanism underlying liver damage toxicity of DDT and the relevant effective inhibitors. Hence, the present study was conducted to explore the protective effects of vitamin C (VC) and vitamin E (VE) on the cytotoxicity of DDT in HL-7702 cells and elaborate the specific molecular mechanisms. The results demonstrated that p,p′-DDT exposure at over 10 µM depleted cell viability of HL-7702 cells and led to cell apoptotic. p,p′-DDT treatment elevated the level of reactive oxygen species (ROS) generation, induced mitochondrial membrane potential, and released cytochrome c into the cytosol, with subsequent elevations of Bax and p53, along with suppression of Bcl-2. In addition, the activations of caspase-3 and -8 were triggered. Furthermore, p,p′-DDT promoted the expressions of NF-κB and FasL. When the cells were exposed to the NF-κB inhibitor (PDTC), the up-regulated expression of FasL was attenuated. Strikingly, these alterations caused by DDT treatment were prevented or reversed by the addition of VC or VE, and the protective effects of co-treatment with VC and VE were higher than the single supplement with p,p′-DDT. Taken together, these findings provide novel experimental evidences supporting that VC or/and VE could reduce p,p′-DDT-induced cytotoxicity of HL-7702 cells via the ROS-mediated mitochondrial pathway and NF-κB/FasL pathway.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0113257en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4252254/pdf/en
dash.licenseLAAen_US
dc.subjectBiology and Life Sciencesen
dc.subjectCell Biologyen
dc.subjectToxicologyen
dc.subjectEcology and Environmental Sciencesen
dc.subjectEnvironmental Protectionen
dc.titleProtective Efficacy of Vitamins C and E on p,p′-DDT-Induced Cytotoxicity via the ROS-Mediated Mitochondrial Pathway and NF-κB/FasL Pathwayen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorCheng, Longen_US
dc.date.available2015-01-05T18:27:35Z
dc.identifier.doi10.1371/journal.pone.0113257*
dash.contributor.affiliatedCheng, Long


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record