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dc.contributor.authorAllen, Eliezer M. Vanen_US
dc.contributor.authorWagle, Nikhilen_US
dc.contributor.authorStojanov, Petaren_US
dc.contributor.authorPerrin, Danielle L.en_US
dc.contributor.authorCibulskis, Kristianen_US
dc.contributor.authorMarlow, Saraen_US
dc.contributor.authorJane-Valbuena, Juditen_US
dc.contributor.authorFriedrich, Dennis C.en_US
dc.contributor.authorKryukov, Gregoryen_US
dc.contributor.authorCarter, Scott L.en_US
dc.contributor.authorMcKenna, Aaronen_US
dc.contributor.authorSivachenko, Andreyen_US
dc.contributor.authorRosenberg, Maraen_US
dc.contributor.authorKiezun, Adamen_US
dc.contributor.authorVoet, Douglasen_US
dc.contributor.authorLawrence, Michaelen_US
dc.contributor.authorLichtenstein, Lee T.en_US
dc.contributor.authorGentry, Jeff G.en_US
dc.contributor.authorHuang, Franklin W.en_US
dc.contributor.authorFostel, Jenniferen_US
dc.contributor.authorFarlow, Deborahen_US
dc.contributor.authorBarbie, Daviden_US
dc.contributor.authorGandhi, Leenaen_US
dc.contributor.authorLander, Eric S.en_US
dc.contributor.authorGray, Stacy W.en_US
dc.contributor.authorJoffe, Stevenen_US
dc.contributor.authorJanne, Pasien_US
dc.contributor.authorGarber, Judyen_US
dc.contributor.authorMacConaill, Lauraen_US
dc.contributor.authorLindeman, Nealen_US
dc.contributor.authorRollins, Barretten_US
dc.contributor.authorKantoff, Philipen_US
dc.contributor.authorFisher, Sheila A.en_US
dc.contributor.authorGabriel, Staceyen_US
dc.contributor.authorGetz, Gaden_US
dc.contributor.authorGarraway, Levi A.en_US
dc.date.accessioned2015-01-05T18:27:49Z
dc.date.issued2013en_US
dc.identifier.citationAllen, E. M. V., N. Wagle, P. Stojanov, D. L. Perrin, K. Cibulskis, S. Marlow, J. Jane-Valbuena, et al. 2013. “Whole-exome sequencing and clinical interpretation of FFPE tumor samples to guide precision cancer medicine.” Nature medicine 20 (6): 682-688. doi:10.1038/nm.3559. http://dx.doi.org/10.1038/nm.3559.en
dc.identifier.issn1078-8956en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13581165
dc.description.abstractTranslating whole exome sequencing (WES) for prospective clinical use may impact the care of cancer patients; however, multiple innovations are necessary for clinical implementation. These include: (1) rapid and robust WES from formalin-fixed paraffin embedded (FFPE) tumor tissue, (2) analytical output similar to data from frozen samples, and (3) clinical interpretation of WES data for prospective use. Here, we describe a prospective clinical WES platform for archival FFPE tumor samples. The platform employs computational methods for effective clinical analysis and interpretation of WES data. When applied retrospectively to 511 exomes, the interpretative framework revealed a “long tail” of somatic alterations in clinically important genes. Prospective application of this approach identified clinically relevant alterations in 15/16 patients. In one patient, previously undetected findings guided clinical trial enrollment leading to an objective clinical response. Overall, this methodology may inform the widespread implementation of precision cancer medicine.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nm.3559en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4048335/pdf/en
dash.licenseLAAen_US
dc.titleWhole-exome sequencing and clinical interpretation of FFPE tumor samples to guide precision cancer medicineen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature medicineen
dash.depositing.authorWagle, Nikhilen_US
dc.date.available2015-01-05T18:27:49Z
dc.identifier.doi10.1038/nm.3559*
dash.authorsorderedfalse
dash.contributor.affiliatedGandhi, Leena
dash.contributor.affiliatedRollins, Barrett
dash.contributor.affiliatedBarbie, David
dash.contributor.affiliatedLindeman, Neal
dash.contributor.affiliatedGray, Stacy
dash.contributor.affiliatedHuang, Franklin
dash.contributor.affiliatedJane-Valbuena, Judit
dash.contributor.affiliatedKantoff, Philip
dash.contributor.affiliatedGarber, Judy
dash.contributor.affiliatedMacConaill, Laura
dash.contributor.affiliatedWagle, Nikhil
dash.contributor.affiliatedJanne, Pasi
dash.contributor.affiliatedGarraway, Levi
dash.contributor.affiliatedGetz, Gad
dash.contributor.affiliatedLander, Eric


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