Fast and Efficient Neural Conversion of Human Hematopoietic Cells
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Author
Castaño, Julio
Menendez, Pablo
Bruzos-Cidon, Cristina
Straccia, Marco
Sousa, Amaia
Zabaleta, Lorea
Vazquez, Nerea
Zubiarrain, Amaia
Ugedo, Luisa
Carvajal-Vergara, Xonia
Canals, Josep Maria
Torrecilla, Maria
Sanchez-Pernaute, Rosario
Giorgetti, Alessandra
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1016/j.stemcr.2014.10.008Metadata
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Castaño, J., P. Menendez, C. Bruzos-Cidon, M. Straccia, A. Sousa, L. Zabaleta, N. Vazquez, et al. 2014. “Fast and Efficient Neural Conversion of Human Hematopoietic Cells.” Stem Cell Reports 3 (6): 1118-1131. doi:10.1016/j.stemcr.2014.10.008. http://dx.doi.org/10.1016/j.stemcr.2014.10.008.Abstract
Summary Neurons obtained directly from human somatic cells hold great promise for disease modeling and drug screening. Available protocols rely on overexpression of transcription factors using integrative vectors and are often slow, complex, and inefficient. We report a fast and efficient approach for generating induced neural cells (iNCs) directly from human hematopoietic cells using Sendai virus. Upon SOX2 and c-MYC expression, CD133-positive cord blood cells rapidly adopt a neuroepithelial morphology and exhibit high expansion capacity. Under defined neurogenic culture conditions, they express mature neuronal markers and fire spontaneous action potentials that can be modulated with neurotransmitters. SOX2 and c-MYC are also sufficient to convert peripheral blood mononuclear cells into iNCs. However, the conversion process is less efficient and resulting iNCs have limited expansion capacity and electrophysiological activity upon differentiation. Our study demonstrates rapid and efficient generation of iNCs from hematopoietic cells while underscoring the impact of target cells on conversion efficiency.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4264063/pdf/Terms of Use
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