Genome-Scale DNA Methylation Mapping of Clinical Samples at Single-Nucleotide Resolution

DSpace/Manakin Repository

Genome-Scale DNA Methylation Mapping of Clinical Samples at Single-Nucleotide Resolution

Citable link to this page

 

 
Title: Genome-Scale DNA Methylation Mapping of Clinical Samples at Single-Nucleotide Resolution
Author: Gu, Hongcang; Bock, Christoph; Mikkelsen, Tarjei S; Jäger, Natalie; Smith, Zachary D; Tomazou, Eleni; Gnirke, Andreas; Lander, Eric Steven; Meissner, Alexander

Note: Order does not necessarily reflect citation order of authors.

Citation: Gu, Hongcang, Christoph Bock, Tarjei S. Mikkelsen, Natalie Jäger, Zachary D. Smith, Eleni Tomazou, Andreas Gnirke, Eric Steven Lander, and Alexander Meissner. 2010. “Genome-Scale DNA Methylation Mapping of Clinical Samples at Single-Nucleotide Resolution.” Nature Methods 7 (2): 133–136.
Full Text & Related Files:
Abstract: Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.
Published Version: doi:10.1038/nmeth.1414
Other Sources: http://www.ncbi.nlm.nih.gov/pubmed/20062050
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:13606766
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters