Genome-Scale DNA Methylation Mapping of Clinical Samples at Single-Nucleotide Resolution
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Author
Gu, Hongcang
Bock, Christoph
Mikkelsen, Tarjei S
Jäger, Natalie
Tomazou, Eleni
Gnirke, Andreas
Published Version
https://doi.org/10.1038/nmeth.1414Metadata
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Gu, Hongcang, Christoph Bock, Tarjei S. Mikkelsen, Natalie Jäger, Zachary D. Smith, Eleni Tomazou, Andreas Gnirke, Eric Steven Lander, and Alexander Meissner. 2010. “Genome-Scale DNA Methylation Mapping of Clinical Samples at Single-Nucleotide Resolution.” Nature Methods 7 (2): 133–136.Abstract
Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.Other Sources
http://www.ncbi.nlm.nih.gov/pubmed/20062050Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:13606766
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