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dc.contributor.authorGu, Hongcang
dc.contributor.authorBock, Christoph
dc.contributor.authorMikkelsen, Tarjei S
dc.contributor.authorJäger, Natalie
dc.contributor.authorSmith, Zachary D
dc.contributor.authorTomazou, Eleni
dc.contributor.authorGnirke, Andreas
dc.contributor.authorLander, Eric Steven
dc.contributor.authorMeissner, Alexander
dc.date.accessioned2015-01-12T19:43:08Z
dc.date.issued2010
dc.identifier.citationGu, Hongcang, Christoph Bock, Tarjei S. Mikkelsen, Natalie Jäger, Zachary D. Smith, Eleni Tomazou, Andreas Gnirke, Eric Steven Lander, and Alexander Meissner. 2010. “Genome-Scale DNA Methylation Mapping of Clinical Samples at Single-Nucleotide Resolution.” Nature Methods 7 (2): 133–136.en_US
dc.identifier.issn1548-7091en_US
dc.identifier.issn1548-7105en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13606766
dc.description.abstractBisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples.en_US
dc.description.sponsorshipStem Cell and Regenerative Biologyen_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofdoi:10.1038/nmeth.1414en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pubmed/20062050en_US
dash.licenseLAA
dc.titleGenome-Scale DNA Methylation Mapping of Clinical Samples at Single-Nucleotide Resolutionen_US
dc.typeJournal Articleen_US
dc.description.versionAuthor's Originalen_US
dc.relation.journalNature Methodsen_US
dash.depositing.authorMeissner, Alexander
dash.waiver2009-12-10
dc.date.available2015-01-12T19:43:08Z
dc.identifier.doi10.1038/nmeth.1414*
dash.contributor.affiliatedSmith, Zachary
dash.contributor.affiliatedLander, Eric
dash.contributor.affiliatedMeissner, Alexander


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