dc.contributor.author | Chao, Luke H | en_US |
dc.contributor.author | Klein, Daryl E | en_US |
dc.contributor.author | Schmidt, Aaron G | en_US |
dc.contributor.author | Peña, Jennifer M | en_US |
dc.contributor.author | Harrison, Stephen C | en_US |
dc.date.accessioned | 2015-02-02T15:31:31Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Chao, Luke H, Daryl E Klein, Aaron G Schmidt, Jennifer M Peña, and Stephen C Harrison. 2014. “Sequential conformational rearrangements in flavivirus membrane fusion.” eLife 3 (1): e04389. doi:10.7554/eLife.04389. http://dx.doi.org/10.7554/eLife.04389. | en |
dc.identifier.issn | 2050-084X | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:13890587 | |
dc.description.abstract | The West Nile Virus (WNV) envelope protein, E, promotes membrane fusion during viral cell entry by undergoing a low-pH triggered conformational reorganization. We have examined the mechanism of WNV fusion and sought evidence for potential intermediates during the conformational transition by following hemifusion of WNV virus-like particles (VLPs) in a single particle format. We have introduced specific mutations into E, to relate their influence on fusion kinetics to structural features of the protein. At the level of individual E subunits, trimer formation and membrane engagement of the threefold clustered fusion loops are rate-limiting. Hemifusion requires at least two adjacent trimers. Simulation of the kinetics indicates that availability of competent monomers within the contact zone between virus and target membrane makes trimerization a bottleneck in hemifusion. We discuss the implications of the model we have derived for mechanisms of membrane fusion in other contexts. DOI: http://dx.doi.org/10.7554/eLife.04389.001 | en |
dc.language.iso | en_US | en |
dc.publisher | eLife Sciences Publications, Ltd | en |
dc.relation.isversionof | doi:10.7554/eLife.04389 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4293572/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | membrane fusion | en |
dc.subject | flavivirus | en |
dc.subject | West Nile virus | en |
dc.subject | single particle | en |
dc.subject | kinetics | en |
dc.subject | conformational change | en |
dc.subject | viruses | en |
dc.title | Sequential conformational rearrangements in flavivirus membrane fusion | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | eLife | en |
dash.depositing.author | Chao, Luke H | en_US |
dc.date.available | 2015-02-02T15:31:31Z | |
dc.identifier.doi | 10.7554/eLife.04389 | * |
dash.contributor.affiliated | Pena, Jennifer | |
dash.contributor.affiliated | Schmidt, Aaron | |
dash.contributor.affiliated | Chao, Luke | |
dash.contributor.affiliated | Klein, Daryl | |
dash.contributor.affiliated | Harrison, Stephen | |