Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas

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Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas

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Title: Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas
Author: Shahrabi-Farahani, Shokoufeh; Wang, Lili; Zwaans, Bernadette M. M.; Santana, Jeans M.; Shimizu, Akio; Takashima, Seiji; Kreuter, Michael; Coultas, Leigh; D'Amore, Patricia A.; Arbeit, Jeffrey M.; Akslen, Lars A.; Bielenberg, Diane R.

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Citation: Shahrabi-Farahani, S., L. Wang, B. M. M. Zwaans, J. M. Santana, A. Shimizu, S. Takashima, M. Kreuter, et al. 2014. “Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.” Laboratory investigation; a journal of technical methods and pathology 94 (7): 752-765. doi:10.1038/labinvest.2014.66. http://dx.doi.org/10.1038/labinvest.2014.66.
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Abstract: Neuropilins (NRP) are cell surface receptors for VEGF and SEMA3 family members. The role of NRP in neurons and endothelial cells has been investigated, but the expression and role of NRP in epithelial cells is much less clear. Herein, the expression and localization of neuropilin 1 (NRP1) was investigated in human and mouse skin and squamous cell carcinomas (SCC). Results indicated that NRP1 mRNA and protein was expressed in the suprabasal epithelial layers of skin sections. NRP1 staining did not overlap with that of keratin 14 (K14) or proliferating cell nuclear antigen, but did colocalize with staining for keratin 1, indicating that differentiated keratinocytes express NRP1. Similar to the expression of NRP1, VEGF-A was expressed in suprabasal epithelial cells, whereas Nrp2 and VEGFR2 were not detectable in the epidermis. The expression of NRP1 correlated with a high degree of differentiation in human SCC specimens, human SCC xenografts, and mouse K14-HPV16 transgenic SCC. UVB irradiation of mouse skin induced Nrp1 upregulation. In vitro, Nrp1 was upregulated in primary keratinocytes in response to differentiating media or EGF-family growth factors. In conclusion, the expression of NRP1 is regulated in the skin and is selectively produced in differentiated epithelial cells. NRP1 may function as a reservoir to sequester VEGF ligand within the epithelial compartment, thereby modulating its bioactivity.
Published Version: doi:10.1038/labinvest.2014.66
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074450/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:13890669
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