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dc.contributor.authorMcClory, Hollisen_US
dc.contributor.authorWilliams, Danaen_US
dc.contributor.authorSapp, Ellenen_US
dc.contributor.authorGatune, Leah Wen_US
dc.contributor.authorWang, Pingen_US
dc.contributor.authorDiFiglia, Marianen_US
dc.contributor.authorLi, Xueyien_US
dc.date.accessioned2015-02-02T15:32:59Z
dc.date.issued2014en_US
dc.identifier.citationMcClory, Hollis, Dana Williams, Ellen Sapp, Leah W Gatune, Ping Wang, Marian DiFiglia, and Xueyi Li. 2014. “Glucose transporter 3 is a rab11-dependent trafficking cargo and its transport to the cell surface is reduced in neurons of CAG140 Huntington’s disease mice.” Acta Neuropathologica Communications 2 (1): 179. doi:10.1186/s40478-014-0178-7. http://dx.doi.org/10.1186/s40478-014-0178-7.en
dc.identifier.issn2051-5960en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:13890720
dc.description.abstractHuntington’s disease (HD) disturbs glucose metabolism in the brain by poorly understood mechanisms. HD neurons have defective glucose uptake, which is attenuated upon enhancing rab11 activity. Rab11 regulates numerous receptors and transporters trafficking onto cell surfaces; its diminished activity in HD cells affects the recycling of transferrin receptor and neuronal glutamate/cysteine transporter EAAC1. Glucose transporter 3 (Glut3) handles most glucose uptake in neurons. Here we investigated rab11 involvement in Glut3 trafficking. Glut3 was localized to rab11 positive puncta in primary neurons and immortalized striatal cells by immunofluorescence labeling and detected in rab11-enriched endosomes immuno-isolated from mouse brain by Western blot. Expression of dominant active and negative rab11 mutants in clonal striatal cells altered the levels of cell surface Glut3 suggesting a regulation by rab11. About 4% of total Glut3 occurred at the cell surface of primary WT neurons. HD140Q/140Q neurons had significantly less cell surface Glut3 than did WT neurons. Western blot analysis revealed comparable levels of Glut3 in the striatum and cortex of WT and HD140Q/140Q mice. However, brain slices immunolabeled with an antibody recognizing an extracellular epitope to Glut3 showed reduced surface expression of Glut3 in the striatum and cortex of HD140Q/140Q mice compared to that of WT mice. Surface labeling of GABAα1 receptor, which is not dependent on rab11, was not different between WT and HD140Q/140Q mouse brain slices. These data define Glut3 to be a rab11-dependent trafficking cargo and suggest that impaired Glut3 trafficking arising from rab11 dysfunction underlies the glucose hypometabolism observed in HD.en
dc.language.isoen_USen
dc.publisherBioMed Centralen
dc.relation.isversionofdoi:10.1186/s40478-014-0178-7en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297405/pdf/en
dash.licenseLAAen_US
dc.subjectHuntington’s diseaseen
dc.subjectGlucose transporter 3en
dc.subjectRab11en
dc.subjectRecycling endosomesen
dc.titleGlucose transporter 3 is a rab11-dependent trafficking cargo and its transport to the cell surface is reduced in neurons of CAG140 Huntington’s disease miceen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalActa Neuropathologica Communicationsen
dash.depositing.authorDiFiglia, Marianen_US
dc.date.available2015-02-02T15:32:59Z
dc.identifier.doi10.1186/s40478-014-0178-7*
dash.contributor.affiliatedLi, Xueyi
dash.contributor.affiliatedDiFiglia, Marian


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