dc.contributor.author | Cocucci, Emanuele | en_US |
dc.contributor.author | Gaudin, Raphaël | en_US |
dc.contributor.author | Kirchhausen, Tom | en_US |
dc.date.accessioned | 2015-02-02T15:33:17Z | |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Cocucci, Emanuele, Raphaël Gaudin, and Tom Kirchhausen. 2014. “Dynamin recruitment and membrane scission at the neck of a clathrin-coated pit.” Molecular Biology of the Cell 25 (22): 3595-3609. doi:10.1091/mbc.E14-07-1240. http://dx.doi.org/10.1091/mbc.E14-07-1240. | en |
dc.identifier.issn | 1059-1524 | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:13890761 | |
dc.description.abstract | Dynamin, the GTPase required for clathrin-mediated endocytosis, is recruited to clathrin-coated pits in two sequential phases. The first is associated with coated pit maturation; the second, with fission of the membrane neck of a coated pit. Using gene-edited cells that express dynamin2-EGFP instead of dynamin2 and live-cell TIRF imaging with single-molecule EGFP sensitivity and high temporal resolution, we detected the arrival of dynamin at coated pits and defined dynamin dimers as the preferred assembly unit. We also used live-cell spinning-disk confocal microscopy calibrated by single-molecule EGFP detection to determine the number of dynamins recruited to the coated pits. A large fraction of budding coated pits recruit between 26 and 40 dynamins (between 1 and 1.5 helical turns of a dynamin collar) during the recruitment phase associated with neck fission; 26 are enough for coated vesicle release in cells partially depleted of dynamin by RNA interference. We discuss how these results restrict models for the mechanism of dynamin-mediated membrane scission. | en |
dc.language.iso | en_US | en |
dc.publisher | The American Society for Cell Biology | en |
dc.relation.isversionof | doi:10.1091/mbc.E14-07-1240 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230619/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | Membrane Trafficking | en |
dc.title | Dynamin recruitment and membrane scission at the neck of a clathrin-coated pit | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | Molecular Biology of the Cell | en |
dash.depositing.author | Cocucci, Emanuele | en_US |
dc.date.available | 2015-02-02T15:33:17Z | |
dc.identifier.doi | 10.1091/mbc.E14-07-1240 | * |
dash.contributor.affiliated | Cocucci, Emanuele | |
dash.contributor.affiliated | Gaudin, Raphaël | |