Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells

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Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells

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Title: Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells
Author: Huh, Sung Jin; Clement, Kendell; Jee, David; Merlini, Alessandra; Choudhury, Sibgat; Maruyama, Reo; Yoo, Ronnie; Chytil, Anna; Boyle, Patrick; Ran, Fei Ann; Moses, Harold L.; Barcellos-Hoff, Mary Helen; Jackson-Grusby, Laurie; Meissner, Alexander; Polyak, Kornelia

Note: Order does not necessarily reflect citation order of authors.

Citation: Huh, S., K. Clement, D. Jee, A. Merlini, S. Choudhury, R. Maruyama, R. Yoo, et al. 2015. “Age- and Pregnancy-Associated DNA Methylation Changes in Mammary Epithelial Cells.” Stem Cell Reports 4 (2): 297-311. doi:10.1016/j.stemcr.2014.12.009. http://dx.doi.org/10.1016/j.stemcr.2014.12.009.
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Abstract: Summary Postnatal mammary gland development and differentiation occur during puberty and pregnancy. To explore the role of DNA methylation in these processes, we determined the genome-wide DNA methylation and gene expression profiles of CD24+CD61+CD29hi, CD24+CD61+CD29lo, and CD24+CD61−CD29lo cell populations that were previously associated with distinct biological properties at different ages and reproductive stages. We found that pregnancy had the most significant effects on CD24+CD61+CD29hi and CD24+CD61+CD29lo cells, inducing distinct epigenetic states that were maintained through life. Integrated analysis of gene expression, DNA methylation, and histone modification profiles revealed cell-type- and reproductive-stage-specific changes. We identified p27 and TGFβ signaling as key regulators of CD24+CD61+CD29lo cell proliferation, based on their expression patterns and results from mammary gland explant cultures. Our results suggest that relatively minor changes in DNA methylation occur during luminal differentiation compared with the effects of pregnancy on CD24+CD61+CD29hi and CD24+CD61+CD29lo cells.
Published Version: doi:10.1016/j.stemcr.2014.12.009
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325231/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:14065312
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