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dc.contributor.authorGrossmann, Nina
dc.contributor.authorVakkasoglu, Ahmet Selim
dc.contributor.authorHulpke, Sabine
dc.contributor.authorAbele, Rupert
dc.contributor.authorGaudet, Rachelle
dc.contributor.authorTampé, Robert
dc.date.accessioned2015-03-12T18:54:20Z
dc.date.issued2014
dc.identifier.citationGrossmann, Nina, Ahmet Selim Vakkasoglu, Sabine Hulpke, Rupert Abele, Rachelle Gaudet, and Robert Tampé. 2014. "Mechanistic Determinants of the Directionality and Energetics of Active Export by a Heterodimeric ABC Transporter." Nature Communications 5:5419.en_US
dc.identifier.issn2041-1723en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:14121878
dc.description.abstractThe ATP-binding cassette (ABC) transporter associated with antigen processing (TAP) participates in immune surveillance by moving proteasomal products into the endoplasmic reticulum (ER) lumen for major histocompatibility complex class I loading and cell surface presentation to cytotoxic T cells. Here we delineate the mechanistic basis for antigen translocation. Notably, TAP works as a molecular diode, translocating peptide substrates against the gradient in a strict unidirectional way. We reveal the importance of the D-loop at the dimer interface of the two nucleotide-binding domains (NBDs) in coupling substrate translocation with ​ATP hydrolysis and defining transport vectoriality. Substitution of the conserved ​aspartate, which coordinates the ATP-binding site, decreases NBD dimerization affinity and turns the unidirectional primary active pump into a passive bidirectional nucleotide-gated facilitator. Thus, ​ATP hydrolysis is not required for translocation per se, but is essential for both active and unidirectional transport. Our data provide detailed mechanistic insight into how heterodimeric ABC exporters operate.en_US
dc.description.sponsorshipMolecular and Cellular Biologyen_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofdoi:10.1038/ncomms6419en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pubmed/25377891en_US
dash.licenseLAA
dc.subjectImmunologyen_US
dc.subjectATPen_US
dc.subjectTransporten_US
dc.subjectBiochemistryen_US
dc.subjectCrystallographyen_US
dc.titleMechanistic Determinants of the Directionality and Energetics of Active Export by a Heterodimeric ABC Transporteren_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalNature Communicationsen_US
dash.depositing.authorGaudet, Rachelle
dc.date.available2015-03-12T18:54:20Z
dc.identifier.doi10.1038/ncomms6419*
workflow.legacycommentscan post pub per sherpa... I think it's a CC-BY work.en_US
dash.contributor.affiliatedVakkasoglu, Ahmet
dash.contributor.affiliatedGaudet, Rachelle


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