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dc.contributor.authorBaron, David M.
dc.contributor.authorClerte, Maeva
dc.contributor.authorBrouckaert, Peter
dc.contributor.authorRaher, Michael J.
dc.contributor.authorFlynn, Aidan W.
dc.contributor.authorZhang, Haihua
dc.contributor.authorCarter, Edward Albert
dc.contributor.authorPicard, Michael Howard
dc.contributor.authorBloch, Kenneth Daniel
dc.contributor.authorBuys, Emmanuel
dc.contributor.authorScherrer-Crosbie, Marielle
dc.date.accessioned2015-03-18T18:12:27Z
dc.date.issued2012
dc.identifier.citationBaron, David M., Maeva Clerte, Peter Brouckaert, Michael J. Raher, Aidan W. Flynn, Haihua Zhang, Edward A. Carter, et al. 2012. In Vivo Noninvasive Characterization of Brown Adipose Tissue Blood Flow by Contrast Ultrasound in Mice. Circulation: Cardiovascular Imaging 5, no. 5: 652–659. Open manuscript version available here: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471995/en_US
dc.identifier.issn1941-9651en_US
dc.identifier.issn1942-0080en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:14229234
dc.description.abstractBackground—Interventions to increase brown adipose tissue (BAT) volume and activation are being extensively investigated as therapies to decrease the body weight in obese subjects. Noninvasive methods to monitor these therapies in animal models and humans are rare. We investigated whether contrast ultrasound (CU) performed in mice could detect BAT and measure its activation by monitoring BAT blood flow. After validation, CU was used to study the role of uncoupling protein 1 and nitric oxide synthases in the acute regulation of BAT blood flow. Methods and Results—Blood flow of interscapular BAT was assessed in mice (n=64) with CU by measuring the signal intensity of continuously infused contrast microbubbles. Blood flow of BAT estimated by CU was 0.5±0.1 (mean±SEM) dB/s at baseline and increased 15-fold during BAT stimulation by norepinephrine (1 µg·kg−1·min−1). Assessment of BAT blood flow using CU was correlated to that performed with fluorescent microspheres (R2=0.86, P<0.001). To evaluate whether intact BAT activation is required to increase BAT blood flow, CU was performed in uncoupling protein 1–deficient mice with impaired BAT activation. Norepinephrine infusion induced a smaller increase in BAT blood flow in uncoupling protein 1–deficient mice than in wild-type mice. Finally, we investigated whether nitric oxide synthases played a role in acute norepinephrine-induced changes of BAT blood flow. Genetic and pharmacologic inhibition of nitric oxide synthase 3 attenuated the norepinephrine-induced increase in BAT blood flow. Conclusions—These results indicate that CU can detect BAT in mice and estimate BAT blood flow in mice with functional differences in BAT.en_US
dc.language.isoen_USen_US
dc.publisherOvid Technologies (Wolters Kluwer Health)en_US
dc.relation.isversionofdoi:10.1161/CIRCIMAGING.112.975607en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3471995/en_US
dash.licenseMETA_ONLY
dc.subjectbrown adipose tissueen_US
dc.subjectimagingen_US
dc.subjectcontrast ultrasounden_US
dc.subjectuncoupling proteinen_US
dc.subjectnitric oxide synthaseen_US
dc.titleIn Vivo Noninvasive Characterization of Brown Adipose Tissue Blood Flow by Contrast Ultrasound in Miceen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalCirculation: Cardiovascular Imagingen_US
dash.depositing.authorBuys, Emmanuel
dash.embargo.until10000-01-01
dc.identifier.doi10.1161/CIRCIMAGING.112.975607*
workflow.legacycommentsFLAG2 publisher grants permission to post only to authors whose institution requires it. Must post dark - but linked to open version on PMC.en_US
dash.authorsorderedfalse
dash.contributor.affiliatedCarter, Edward
dash.contributor.affiliatedPicard, Michael
dash.contributor.affiliatedScherrer-Crosbie, Marielle
dash.contributor.affiliatedBuys, Emmanuel
dash.contributor.affiliatedBloch, Kenneth


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