Neuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells
View/ Open
Author
Wang, Hong-Bo
Zhang, Hua
Zhang, Jing-Ping
Li, Yan
Feng, Guo-Kai
Du, Yong
Xiong, Dan
Zhong, Qian
Liu, Wan-Li
Du, Huamao
Li, Man-Zhi
Huang, Wen-Lin
Tsao, Sai Wah
Hutt-Fletcher, Lindsey
Zeng, Yi-Xin
Zeng, Mu-Sheng
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1038/ncomms7240Metadata
Show full item recordCitation
Wang, H., H. Zhang, J. Zhang, Y. Li, B. Zhao, G. Feng, Y. Du, et al. 2015. “Neuropilin 1 is an entry factor that promotes EBV infection of nasopharyngeal epithelial cells.” Nature Communications 6 (1): 6240. doi:10.1038/ncomms7240. http://dx.doi.org/10.1038/ncomms7240.Abstract
Epstein–Barr virus (EBV) is implicated as an aetiological factor in B lymphomas and nasopharyngeal carcinoma. The mechanisms of cell-free EBV infection of nasopharyngeal epithelial cells remain elusive. EBV glycoprotein B (gB) is the critical fusion protein for infection of both B and epithelial cells, and determines EBV susceptibility of non-B cells. Here we show that neuropilin 1 (NRP1) directly interacts with EBV gB23–431. Either knockdown of NRP1 or pretreatment of EBV with soluble NRP1 suppresses EBV infection. Upregulation of NRP1 by overexpression or EGF treatment enhances EBV infection. However, NRP2, the homologue of NRP1, impairs EBV infection. EBV enters nasopharyngeal epithelial cells through NRP1-facilitated internalization and fusion, and through macropinocytosis and lipid raft-dependent endocytosis. NRP1 partially mediates EBV-activated EGFR/RAS/ERK signalling, and NRP1-dependent receptor tyrosine kinase (RTK) signalling promotes EBV infection. Taken together, NRP1 is identified as an EBV entry factor that cooperatively activates RTK signalling, which subsequently promotes EBV infection in nasopharyngeal epithelial cells.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339892/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:14351058
Collections
- FAS Scholarly Articles [18256]
- HMS Scholarly Articles [17917]
Contact administrator regarding this item (to report mistakes or request changes)