Chromosomal attachments set length and microtubule number in the Saccharomyces cerevisiae mitotic spindle

DSpace/Manakin Repository

Chromosomal attachments set length and microtubule number in the Saccharomyces cerevisiae mitotic spindle

Citable link to this page

 

 
Title: Chromosomal attachments set length and microtubule number in the Saccharomyces cerevisiae mitotic spindle
Author: Nannas, Natalie J.; O’Toole, Eileen T.; Winey, Mark; Murray, Andrew W.

Note: Order does not necessarily reflect citation order of authors.

Citation: Nannas, Natalie J., Eileen T. O’Toole, Mark Winey, and Andrew W. Murray. 2014. “Chromosomal attachments set length and microtubule number in the Saccharomyces cerevisiae mitotic spindle.” Molecular Biology of the Cell 25 (25): 4034-4048. doi:10.1091/mbc.E14-01-0016. http://dx.doi.org/10.1091/mbc.E14-01-0016.
Full Text & Related Files:
Abstract: The length of the mitotic spindle varies among different cell types. A simple model for spindle length regulation requires balancing two forces: pulling, due to micro­tubules that attach to the chromosomes at their kinetochores, and pushing, due to interactions between microtubules that emanate from opposite spindle poles. In the budding yeast Saccharomyces cerevisiae, we show that spindle length scales with kinetochore number, increasing when kinetochores are inactivated and shortening on addition of synthetic or natural kinetochores, showing that kinetochore–microtubule interactions generate an inward force to balance forces that elongate the spindle. Electron microscopy shows that manipulating kinetochore number alters the number of spindle microtubules: adding extra kinetochores increases the number of spindle microtubules, suggesting kinetochore-based regulation of microtubule number.
Published Version: doi:10.1091/mbc.E14-01-0016
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4263447/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:14351189
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters