Intravital Microscopic Interrogation of Peripheral Taste Sensation
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Author
Choi, Myunghwan
Lee, Woei Ming
Yun, Seok Hyun
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https://doi.org/10.1038/srep08661Metadata
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Choi, Myunghwan, Woei Ming Lee, and Seok Hyun Yun. 2015. “Intravital Microscopic Interrogation of Peripheral Taste Sensation.” Scientific Reports 5 (1): 8661. doi:10.1038/srep08661. http://dx.doi.org/10.1038/srep08661.Abstract
Intravital microscopy is a powerful tool in neuroscience but has not been adapted to the taste sensory organ due to anatomical constraint. Here we developed an imaging window to facilitate microscopic access to the murine tongue in vivo. Real-time two-photon microscopy allowed the visualization of three-dimensional microanatomy of the intact tongue mucosa and functional activity of taste cells in response to topically administered tastants in live mice. Video microscopy also showed the calcium activity of taste cells elicited by small-sized tastants in the blood circulation. Molecular kinetic analysis suggested that intravascular taste sensation takes place at the microvilli on the apical side of taste cells after diffusion of the molecules through the pericellular capillaries and tight junctions in the taste bud. Our results demonstrate the capabilities and utilities of the new tool for taste research in vivo.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4345348/pdf/Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:14351230
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