Pathogenic E. coli Exploits SslE Mucinase Activity to Translocate through the Mucosal Barrier and Get Access to Host Cells

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Pathogenic E. coli Exploits SslE Mucinase Activity to Translocate through the Mucosal Barrier and Get Access to Host Cells

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Title: Pathogenic E. coli Exploits SslE Mucinase Activity to Translocate through the Mucosal Barrier and Get Access to Host Cells
Author: Valeri, Maria; Rossi Paccani, Silvia; Kasendra, Magdalena; Nesta, Barbara; Serino, Laura; Pizza, Mariagrazia; Soriani, Marco

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Citation: Valeri, Maria, Silvia Rossi Paccani, Magdalena Kasendra, Barbara Nesta, Laura Serino, Mariagrazia Pizza, and Marco Soriani. 2015. “Pathogenic E. coli Exploits SslE Mucinase Activity to Translocate through the Mucosal Barrier and Get Access to Host Cells.” PLoS ONE 10 (3): e0117486. doi:10.1371/journal.pone.0117486. http://dx.doi.org/10.1371/journal.pone.0117486.
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Abstract: SslE is a zinc-metalloprotease involved in the degradation of mucin substrates and recently proposed as a potential vaccine candidate against pathogenic E. coli. In this paper, by exploiting a human in vitro model of mucus-secreting cells, we demonstrated that bacteria expressing SslE have a metabolic benefit which results in an increased growth rate postulating the importance of this antigen in enhancing E. coli fitness. We also observed that SslE expression facilitates E. coli penetration of the mucus favouring bacteria adhesion to host cells. Moreover, we found that SslE-mediated opening of the mucosae contributed to the activation of pro-inflammatory events. Indeed, intestinal cells infected with SslE-secreting bacteria showed an increased production of IL-8 contributing to neutrophil recruitment. The results presented in this paper conclusively designate SslE as an important colonization factor favouring E. coli access to both metabolic substrates and target cells.
Published Version: doi:10.1371/journal.pone.0117486
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4366376/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:14351285
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