Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

 
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Sharei, Armon
Jhunjhunwala, Siddharth
Hartoularos, George C.
Eyerman, Alexandra T.
Lytton-Jean, Abigail
Angin, Mathieu
Sharma, Siddhartha
Poceviciute, Roberta
Mao, Shirley
Heimann, Megan
Liu, Sophia
Talkar, Tanya
Khan, Omar F.
Addo, Marylyn
Anderson, Daniel G.
Langer, Robert
Jensen, Klavs F.
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1371/journal.pone.0118803
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Citation
Sharei, A., R. Trifonova, S. Jhunjhunwala, G. C. Hartoularos, A. T. Eyerman, A. Lytton-Jean, M. Angin, et al. 2015. “Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells.” PLoS ONE 10 (4): e0118803. doi:10.1371/journal.pone.0118803. http://dx.doi.org/10.1371/journal.pone.0118803.
Abstract
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395260/pdf/
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This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:15034758

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