Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
Hartoularos, George C.
Eyerman, Alexandra T.
Khan, Omar F.
Anderson, Daniel G.
Jensen, Klavs F.Note: Order does not necessarily reflect citation order of authors.
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CitationSharei, A., R. Trifonova, S. Jhunjhunwala, G. C. Hartoularos, A. T. Eyerman, A. Lytton-Jean, M. Angin, et al. 2015. “Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells.” PLoS ONE 10 (4): e0118803. doi:10.1371/journal.pone.0118803. http://dx.doi.org/10.1371/journal.pone.0118803.
AbstractIntracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.
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