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dc.contributor.authorSharei, Armonen_US
dc.contributor.authorTrifonova, Radianaen_US
dc.contributor.authorJhunjhunwala, Siddharthen_US
dc.contributor.authorHartoularos, George C.en_US
dc.contributor.authorEyerman, Alexandra T.en_US
dc.contributor.authorLytton-Jean, Abigailen_US
dc.contributor.authorAngin, Mathieuen_US
dc.contributor.authorSharma, Siddharthaen_US
dc.contributor.authorPoceviciute, Robertaen_US
dc.contributor.authorMao, Shirleyen_US
dc.contributor.authorHeimann, Meganen_US
dc.contributor.authorLiu, Sophiaen_US
dc.contributor.authorTalkar, Tanyaen_US
dc.contributor.authorKhan, Omar F.en_US
dc.contributor.authorAddo, Marylynen_US
dc.contributor.authorvon Andrian, Ulrich H.en_US
dc.contributor.authorAnderson, Daniel G.en_US
dc.contributor.authorLanger, Roberten_US
dc.contributor.authorLieberman, Judyen_US
dc.contributor.authorJensen, Klavs F.en_US
dc.date.accessioned2015-05-04T15:25:35Z
dc.date.issued2015en_US
dc.identifier.citationSharei, A., R. Trifonova, S. Jhunjhunwala, G. C. Hartoularos, A. T. Eyerman, A. Lytton-Jean, M. Angin, et al. 2015. “Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells.” PLoS ONE 10 (4): e0118803. doi:10.1371/journal.pone.0118803. http://dx.doi.org/10.1371/journal.pone.0118803.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:15034758
dc.description.abstractIntracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0118803en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4395260/pdf/en
dash.licenseLAAen_US
dc.titleEx Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cellsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorTrifonova, Radianaen_US
dc.date.available2015-05-04T15:25:35Z
dc.identifier.doi10.1371/journal.pone.0118803*
dash.authorsorderedfalse
dash.contributor.affiliatedTrifonova, Radiana
dash.contributor.affiliatedLieberman, Judy
dash.contributor.affiliatedvon Andrian-Werburg, Ulrich


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