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dc.contributor.authorBaccarelli, Andrea Aen_US
dc.contributor.authorByun, Hyang-Minen_US
dc.date.accessioned2015-05-04T15:26:22Z
dc.date.issued2015en_US
dc.identifier.citationBaccarelli, Andrea A., and Hyang-Min Byun. 2015. “Platelet mitochondrial DNA methylation: a potential new marker of cardiovascular disease.” Clinical Epigenetics 7 (1): 44. doi:10.1186/s13148-015-0078-0. http://dx.doi.org/10.1186/s13148-015-0078-0.en
dc.identifier.issn1868-7075en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:15034850
dc.description.abstractBackground: Platelets are critical in the etiology of cardiovascular disease (CVD), and the mitochondria in these cells serve as an energy source for platelet function. Epigenetic factors, especially DNA methylation, have been employed as markers of CVD. Unlike nuclear DNA methylation, mitochondrial DNA (mtDNA) methylation has not been widely studied, in part, due to debate about its existence and role. In this study, we examined platelet mtDNA methylation in relation to CVD. Results: We measured mtDNA methylation in platelets by bisulfite-PCR pyrosequencing and examined associations of CVD with methylation in mitochondrial genes; cytochrome c oxidase (MT-CO1, MT-CO2, and MT-CO3); tRNA leucine 1 (MT-TL1); ATP synthase (MT-ATP6 and MT-ATP8); and NADH dehydrogenase (MT-MD5). We report that CVD patients have significantly higher mtDNA methylation than healthy controls in MT-CO1 (18.53%, P < 0.0001), MT-CO2 (3.33%, P = 0.0001), MT-CO3 (0.92%, P < 0.0001), and MT-TL1 (1.67%, P = 0.0001), which are involved in ATP synthesis. Platelet mtDNA methylation was not related with age, BMI, and race in this study. Conclusions: Our results suggest that platelet mtDNA methylation, which could serve as non-invasive and easy-to-obtain markers, may be implicated in the etiology of CVD. Electronic supplementary material The online version of this article (doi:10.1186/s13148-015-0078-0) contains supplementary material, which is available to authorized users.en
dc.language.isoen_USen
dc.publisherBioMed Centralen
dc.relation.isversionofdoi:10.1186/s13148-015-0078-0en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4404685/pdf/en
dash.licenseLAAen_US
dc.subjectMitochondrial epigeneticsen
dc.subjectDNA methylationen
dc.subjectPlateleten
dc.subjectCardiovascular diseaseen
dc.titlePlatelet mitochondrial DNA methylation: a potential new marker of cardiovascular diseaseen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalClinical Epigeneticsen
dash.depositing.authorBaccarelli, Andrea Aen_US
dc.date.available2015-05-04T15:26:22Z
dc.identifier.doi10.1186/s13148-015-0078-0*
dash.contributor.affiliatedBaccarelli, Andrea
dc.identifier.orcid0000-0002-3436-0640


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