Show simple item record

dc.contributor.authorLiu, Xue-Songen_US
dc.contributor.authorLittle, John B.en_US
dc.contributor.authorYuan, Zhi-Minen_US
dc.date.accessioned2015-06-02T12:20:30Z
dc.date.issued2015en_US
dc.identifier.citationLiu, Xue-Song, John B. Little, and Zhi-Min Yuan. 2015. “Glycolytic metabolism influences global chromatin structure.” Oncotarget 6 (6): 4214-4225.en
dc.identifier.issn1949-2553en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:16120909
dc.description.abstractMetabolic rewiring, specifically elevated glycolytic metabolism is a hallmark of cancer. Global chromatin structure regulates gene expression, DNA repair, and also affects cancer progression. But the interrelationship between tumor metabolism and chromatin architecture remain unclear. Here we show that increased glycolysis in cancer cells promotes an open chromatin configuration. Using complementary methods including Micrococcal nuclease (MNase) digestion assay, electron microscope and immunofluorescence staining, we demonstrate that glycolysis inhibition by pharmacological and genetic approaches was associated with induction of compacted chromatin structure. This condensed chromatin status appeared to result chiefly from histone hypoacetylation as restoration of histone acetylation with an HDAC inhibitor reversed the compacted chromatin state. Interestingly, glycolysis inhibition-induced chromatin condensation impeded DNA repair efficiency leading to increased sensitivity of cancer cells to DNA damage drugs, which may represent a novel molecular mechanism that can be exploited for cancer therapy.en
dc.language.isoen_USen
dc.publisherImpact Journals LLCen
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414184/pdf/en
dash.licenseLAAen_US
dc.subjectglycolysisen
dc.subjectacetylationen
dc.subjectchromatin structureen
dc.subjectchemosensitivityen
dc.titleGlycolytic metabolism influences global chromatin structureen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalOncotargeten
dash.depositing.authorLittle, John B.en_US
dc.date.available2015-06-02T12:20:30Z
dc.identifier.doi10.18632/oncotarget.2929
dash.contributor.affiliatedLittle, John
dash.contributor.affiliatedYuan, Zhi-Min


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record