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dc.contributor.authorGlavan, Ana C
dc.contributor.authorChristodouleas, Dionysios
dc.contributor.authorMosadegh, Bobak
dc.contributor.authorYu, Hai Dong
dc.contributor.authorSmith, Barbara S.
dc.contributor.authorLessing, Joshua
dc.contributor.authorFernández-Abedul, M. Teresa
dc.contributor.authorWhitesides, George McClelland
dc.date.accessioned2015-07-01T20:39:53Z
dc.date.issued2014
dc.identifier.citationGlavan, Ana C., Dionysios C. Christodouleas, Bobak Mosadegh, Hai Dong Yu, Barbara S. Smith, Joshua Lessing, M. Teresa Fernández-Abedul, and George M. Whitesides. 2014. “ Folding Analytical Devices for Electrochemical ELISA in Hydrophobic R H Paper .” Analytical Chemistry 86 (24) (December 16): 11999–12007. doi:10.1021/ac5020782.en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:16922668
dc.description.abstractThis work describes a device for electrochemical enzyme-linked immunosorbent assay (ELISA) designed for low-resource settings and diagnostics at the point of care. The device is fabricated entirely in hydrophobic paper, produced by silanization of paper with decyl trichlorosilane, and comprises two zones separated by a central crease: an embossed microwell, on the surface of which the antigen or antibody immobilization and recognition events occur, and a detection zone where the electrodes are printed. The two zones are brought in contact by folding the device along this central crease; the analytical signal is recorded from the folded configuration. Two proof-of-concept applications, an electrochemical direct ELISA for the detection of rabbit IgG as a model antigen in buffer and an electrochemical sandwich ELISA for the detection of malarial histidine-rich protein from Plasmodium falciparum (Pf HRP2) in spiked human serum, show the versatility of this device. The limit of detection of the electrochemical sandwich ELISA for the quantification of Pf HRP2 in spiked human serum was 4 ng mL–1 (102 pmol L–1), a value within the range of clinically relevant concentrations.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.isversionofdoi:10.1021/ac5020782en_US
dash.licenseOAP
dc.titleFolding Analytical Devices for Electrochemical ELISA in Hydrophobic R H Paperen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalAnalytical Chemistryen_US
dash.depositing.authorWhitesides, George McClelland
dc.date.available2015-07-01T20:39:53Z
dc.identifier.doi10.1021/ac5020782*
workflow.legacycommentsFAR 2014en_US
dash.contributor.affiliatedChristodouleas, Dionysios
dash.contributor.affiliatedGlavan, Ana
dash.contributor.affiliatedWhitesides, George


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