A DNA-based molecular probe for optically reporting cellular traction forces

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A DNA-based molecular probe for optically reporting cellular traction forces

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Title: A DNA-based molecular probe for optically reporting cellular traction forces
Author: Blakely, Brandon L.; Dumelin, Christoph E.; Trappmann, Britta; McGregor, Lynn M.; Choi, Colin K.; Anthony, Peter C.; Duesterberg, Van K.; Baker, Brendon M.; Block, Steven M.; Liu, David R.; Chen, Christopher S.

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Citation: Blakely, B. L., C. E. Dumelin, B. Trappmann, L. M. McGregor, C. K. Choi, P. C. Anthony, V. K. Duesterberg, et al. 2014. “A DNA-based molecular probe for optically reporting cellular traction forces.” Nature methods 11 (12): 1229-1232. doi:10.1038/nmeth.3145. http://dx.doi.org/10.1038/nmeth.3145.
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Abstract: We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges.
Published Version: doi:10.1038/nmeth.3145
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4247985/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:17295511
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