Show simple item record

dc.contributor.authorCardarelli, Liaen_US
dc.contributor.authorSaak, Christinaen_US
dc.contributor.authorGibbs, Karine A.en_US
dc.date.accessioned2015-07-13T18:45:43Z
dc.date.issued2015en_US
dc.identifier.citationCardarelli, Lia, Christina Saak, and Karine A. Gibbs. 2015. “Two Proteins Form a Heteromeric Bacterial Self-Recognition Complex in Which Variable Subdomains Determine Allele-Restricted Binding.” mBio 6 (3): e00251-15. doi:10.1128/mBio.00251-15. http://dx.doi.org/10.1128/mBio.00251-15.en
dc.identifier.issn2150-7511en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17295588
dc.description.abstractABSTRACT Self- versus nonself-recognition in bacteria has been described recently through genetic analyses in multiple systems; however, understanding of the biochemical properties and mechanisms of recognition-determinant proteins remains limited. Here we extend the molecular and biochemical understanding of two recognition-determinant proteins in bacteria. We have found that a heterotypic complex is formed between two bacterial self-recognition proteins, IdsD and IdsE, the genes of which have been shown to genetically encode the determinants for strain-specific identity in the opportunistic bacterial pathogen Proteus mirabilis. This IdsD-IdsE complex forms independently of other P. mirabilis-encoded self-recognition proteins. We have also shown that the binding between IdsD and IdsE is strain- and allele-specific. The specificity for interactions is encoded within a predicted membrane-spanning subdomain within each protein that contains stretches of unique amino acids in each P. mirabilis variant. Finally, we have demonstrated that this in vitro IdsD-IdsE binding interaction correlates to in vivo population identity, suggesting that the binding interactions between IdsD and IdsE are part of a cellular pathway that underpins self-recognition behavior in P. mirabilis and drives bacterial population sociality.en
dc.language.isoen_USen
dc.publisherAmerican Society of Microbiologyen
dc.relation.isversionofdoi:10.1128/mBio.00251-15en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471559/pdf/en
dash.licenseLAAen_US
dc.titleTwo Proteins Form a Heteromeric Bacterial Self-Recognition Complex in Which Variable Subdomains Determine Allele-Restricted Bindingen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalmBioen
dash.depositing.authorSaak, Christinaen_US
dc.date.available2015-07-13T18:45:43Z
dc.identifier.doi10.1128/mBio.00251-15*
dash.contributor.affiliatedSaak, Christina
dash.contributor.affiliatedGibbs, Karine


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record