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dc.contributor.authorCurtis, Edward Aen_US
dc.contributor.authorLiu, David Ren_US
dc.date.accessioned2015-07-13T18:45:52Z
dc.date.issued2014en_US
dc.identifier.citationCurtis, Edward A., and David R Liu. 2014. “A naturally occurring, noncanonical GTP aptamer made of simple tandem repeats.” RNA Biology 11 (6): 682-692. doi:10.4161/rna.28798. http://dx.doi.org/10.4161/rna.28798.en
dc.identifier.issn1547-6286en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17295593
dc.description.abstractRecently, we used in vitro selection to identify a new class of naturally occurring GTP aptamer called the G motif. Here we report the discovery and characterization of a second class of naturally occurring GTP aptamer, the “CA motif.” The primary sequence of this aptamer is unusual in that it consists entirely of tandem repeats of CA-rich motifs as short as three nucleotides. Several active variants of the CA motif aptamer lack the ability to form consecutive Watson-Crick base pairs in any register, while others consist of repeats containing only cytidine and adenosine residues, indicating that noncanonical interactions play important roles in its structure. The circular dichroism spectrum of the CA motif aptamer is distinct from that of A-form RNA and other major classes of nucleic acid structures. Bioinformatic searches indicate that the CA motif is absent from most archaeal and bacterial genomes, but occurs in at least 70 percent of approximately 400 eukaryotic genomes examined. These searches also uncovered several phylogenetically conserved examples of the CA motif in rodent (mouse and rat) genomes. Together, these results reveal the existence of a second class of naturally occurring GTP aptamer whose sequence requirements, like that of the G motif, are not consistent with those of a canonical secondary structure. They also indicate a new and unexpected potential biochemical activity of certain naturally occurring tandem repeats.en
dc.language.isoen_USen
dc.publisherLandes Bioscienceen
dc.relation.isversionofdoi:10.4161/rna.28798en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4156500/pdf/en
dash.licenseLAAen_US
dc.subjectnaturally occurring aptameren
dc.subjecttandem repeatsen
dc.subjectin vitro selectionen
dc.subjectGTPen
dc.subjectRNAen
dc.subjectmicrosatelliteen
dc.titleA naturally occurring, noncanonical GTP aptamer made of simple tandem repeatsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalRNA Biologyen
dash.depositing.authorLiu, David Ren_US
dc.date.available2015-07-13T18:45:52Z
dc.identifier.doi10.4161/rna.28798*
dash.contributor.affiliatedLiu, David


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