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dc.contributor.authorPoulter, Natalie S.en_US
dc.contributor.authorPollitt, Alice Y.en_US
dc.contributor.authorDavies, Amyen_US
dc.contributor.authorMalinova, Dessislavaen_US
dc.contributor.authorNash, Gerard B.en_US
dc.contributor.authorHannon, Mike J.en_US
dc.contributor.authorPikramenou, Zoeen_US
dc.contributor.authorRappoport, Joshua Z.en_US
dc.contributor.authorHartwig, John H.en_US
dc.contributor.authorOwen, Dylan M.en_US
dc.contributor.authorThrasher, Adrian J.en_US
dc.contributor.authorWatson, Stephen P.en_US
dc.contributor.authorThomas, Steven G.en_US
dc.date.accessioned2015-07-13T18:46:48Z
dc.date.issued2015en_US
dc.identifier.citationPoulter, N. S., A. Y. Pollitt, A. Davies, D. Malinova, G. B. Nash, M. J. Hannon, Z. Pikramenou, et al. 2015. “Platelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complex.” Nature Communications 6 (1): 7254. doi:10.1038/ncomms8254. http://dx.doi.org/10.1038/ncomms8254.en
dc.identifier.issn2041-1723en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17295613
dc.description.abstractThe actin nodule is a novel F-actin structure present in platelets during early spreading. However, only limited detail is known regarding nodule organization and function. Here we use electron microscopy, SIM and dSTORM super-resolution, and live-cell TIRF microscopy to characterize the structural organization and signalling pathways associated with nodule formation. Nodules are composed of up to four actin-rich structures linked together by actin bundles. They are enriched in the adhesion-related proteins talin and vinculin, have a central core of tyrosine phosphorylated proteins and are depleted of integrins at the plasma membrane. Nodule formation is dependent on Wiskott–Aldrich syndrome protein (WASp) and the ARP2/3 complex. WASp−/− mouse blood displays impaired platelet aggregate formation at arteriolar shear rates. We propose actin nodules are platelet podosome-related structures required for platelet–platelet interaction and their absence contributes to the bleeding diathesis of Wiskott–Aldrich syndrome.en
dc.language.isoen_USen
dc.publisherNature Pub. Groupen
dc.relation.isversionofdoi:10.1038/ncomms8254en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458878/pdf/en
dash.licenseLAAen_US
dc.titlePlatelet actin nodules are podosome-like structures dependent on Wiskott–Aldrich syndrome protein and ARP2/3 complexen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature Communicationsen
dash.depositing.authorHartwig, John H.en_US
dc.date.available2015-07-13T18:46:48Z
dc.identifier.doi10.1038/ncomms8254*
dash.authorsorderedfalse
dash.contributor.affiliatedHartwig, John


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