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dc.contributor.authorFu, Bishien_US
dc.contributor.authorWang, Lingyanen_US
dc.contributor.authorDing, Haoen_US
dc.contributor.authorSchwamborn, Jens C.en_US
dc.contributor.authorLi, Shitaoen_US
dc.contributor.authorDorf, Martin E.en_US
dc.date.accessioned2015-07-13T18:46:58Z
dc.date.issued2015en_US
dc.identifier.citationFu, Bishi, Lingyan Wang, Hao Ding, Jens C. Schwamborn, Shitao Li, and Martin E. Dorf. 2015. “TRIM32 Senses and Restricts Influenza A Virus by Ubiquitination of PB1 Polymerase.” PLoS Pathogens 11 (6): e1004960. doi:10.1371/journal.ppat.1004960. http://dx.doi.org/10.1371/journal.ppat.1004960.en
dc.identifier.issn1553-7366en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17295638
dc.description.abstractPolymerase basic protein 1 (PB1) is the catalytic core of the influenza A virus (IAV) RNA polymerase complex essential for viral transcription and replication. Understanding the intrinsic mechanisms which block PB1 function could stimulate development of new anti-influenza therapeutics. Affinity purification coupled with mass spectrometry (AP-MS) was used to identify host factors interacting with PB1. Among PB1 interactors, the E3 ubiquitin ligase TRIM32 interacts with PB1 proteins derived from multiple IAV strains. TRIM32 senses IAV infection by interacting with PB1 and translocates with PB1 to the nucleus following influenza infection. Ectopic TRIM32 expression attenuates IAV infection. Conversely, RNAi depletion and knockout of TRIM32 increase susceptibility of tracheal and lung epithelial cells to IAV infection. Reconstitution of trim32-/- mouse embryonic fibroblasts with TRIM32, but not a catalytically inactive mutant, restores viral restriction. Furthermore, TRIM32 directly ubiquitinates PB1, leading to PB1 protein degradation and subsequent reduction of polymerase activity. Thus, TRIM32 is an intrinsic IAV restriction factor which senses and targets the PB1 polymerase for ubiquitination and protein degradation. TRIM32 represents a model of intrinsic immunity, in which a host protein directly senses and counters viral infection in a species specific fashion by directly limiting viral replication.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.ppat.1004960en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461266/pdf/en
dash.licenseLAAen_US
dc.titleTRIM32 Senses and Restricts Influenza A Virus by Ubiquitination of PB1 Polymeraseen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS Pathogensen
dash.depositing.authorFu, Bishien_US
dc.date.available2015-07-13T18:46:58Z
dc.identifier.doi10.1371/journal.ppat.1004960*
dash.contributor.affiliatedWang, Lingyan
dash.contributor.affiliatedDorf, Martin
dash.contributor.affiliatedFu, Bishi


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