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dc.contributor.authorCampo, David S.en_US
dc.contributor.authorSkums, Pavelen_US
dc.contributor.authorDimitrova, Zoyaen_US
dc.contributor.authorVaughan, Gilbertoen_US
dc.contributor.authorForbi, Joseph C.en_US
dc.contributor.authorTeo, Chong-Geeen_US
dc.contributor.authorKhudyakov, Yuryen_US
dc.contributor.authorLau, Daryl T.-Y.en_US
dc.date.accessioned2015-07-13T18:47:04Z
dc.date.issued2014en_US
dc.identifier.citationCampo, David S., Pavel Skums, Zoya Dimitrova, Gilberto Vaughan, Joseph C. Forbi, Chong-Gee Teo, Yury Khudyakov, and Daryl T.-Y. Lau. 2014. “Drug-resistance of a viral population and its individual intra-host variants during the first 48 hours of therapy.” Clinical pharmacology and therapeutics 95 (6): 627-635. doi:10.1038/clpt.2014.20. http://dx.doi.org/10.1038/clpt.2014.20.en
dc.identifier.issn0009-9236en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17295655
dc.description.abstractUsing HCV and IFN-resistance as a proof of concept, we have devised a new methodology for calculating the effect of a drug over a viral population and the resistance of its individual intra-host variants. By means of next-generation sequencing, HCV variants were obtained from sera collected at 9 time-points from 16 patients during the first 48 hours after injection of IFN-α. IFN-resistance coefficients were calculated for individual variants using changes in their relative frequencies, and for the entire intra-host viral population using changes in viral titer during the initial 48 hours. Population-wide resistance and presence of IFN-resistant variants were highly associated with pegIFN-α2a/RBV treatment outcome at week 12 (p = 3.78×10-5 and 0.0114, respectively). This new method allows an accurate measurement of resistance based solely on changes in viral titer or the relative frequency of intra-host viral variants during a short observation time.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/clpt.2014.20en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215939/pdf/en
dash.licenseLAAen_US
dc.titleDrug-resistance of a viral population and its individual intra-host variants during the first 48 hours of therapyen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalClinical pharmacology and therapeuticsen
dc.date.available2015-07-13T18:47:04Z
dc.identifier.doi10.1038/clpt.2014.20*


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