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dc.contributor.authorLabunskyy, Vyacheslav M.en_US
dc.contributor.authorSuzuki, Yoen_US
dc.contributor.authorHanly, Timothy J.en_US
dc.contributor.authorMurao, Ayakoen_US
dc.contributor.authorRoth, Frederick P.en_US
dc.contributor.authorGladyshev, Vadim N.en_US
dc.date.accessioned2015-07-13T18:47:30Z
dc.date.issued2014en_US
dc.identifier.citationLabunskyy, Vyacheslav M., Yo Suzuki, Timothy J. Hanly, Ayako Murao, Frederick P. Roth, and Vadim N. Gladyshev. 2014. “The Insertion Green Monster (iGM) Method for Expression of Multiple Exogenous Genes in Yeast.” G3: Genes|Genomes|Genetics 4 (7): 1183-1191. doi:10.1534/g3.114.010868. http://dx.doi.org/10.1534/g3.114.010868.en
dc.identifier.issn2160-1836en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17295719
dc.description.abstractBeing a simple eukaryotic organism, Saccharomyces cerevisiae provides numerous advantages for expression and functional characterization of proteins from higher eukaryotes, including humans. However, studies of complex exogenous pathways using yeast as a host have been hampered by the lack of tools to engineer strains expressing a large number of genetic components. In addition to inserting multiple genes, it is often desirable to knock out or replace multiple endogenous genes that might interfere with the processes studied. Here, we describe the “insertion Green Monster” (iGM) set of expression vectors that enable precise insertion of many heterologous genes into the yeast genome in a rapid and reproducible manner and permit simultaneous replacement of selected yeast genes. As a proof of principle, we have used the iGM method to replace components of the yeast pathway for methionine sulfoxide reduction with genes encoding the human selenoprotein biosynthesis machinery and generated a single yeast strain carrying 11 exogenous components of the selenoprotein biosynthetic pathway in precisely engineered loci.en
dc.language.isoen_USen
dc.publisherGenetics Society of Americaen
dc.relation.isversionofdoi:10.1534/g3.114.010868en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455768/pdf/en
dash.licenseLAAen_US
dc.subjectsynthetic biologyen
dc.subjectmulti-gene insertionsen
dc.subjecten
dc.subjectgreen fluorescent proteinen
dc.subjectflow cytometryen
dc.titleThe Insertion Green Monster (iGM) Method for Expression of Multiple Exogenous Genes in Yeasten
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalG3: Genes|Genomes|Geneticsen
dash.depositing.authorGladyshev, Vadim N.en_US
dc.date.available2015-07-13T18:47:30Z
dc.identifier.doi10.1534/g3.114.010868*
dash.contributor.affiliatedGladyshev, Vadim


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