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dc.contributor.authorKumar, Roshan M.en_US
dc.contributor.authorCahan, Patricken_US
dc.contributor.authorShalek, Alex K.en_US
dc.contributor.authorSatija, Rahulen_US
dc.contributor.authorDaleyKeyser, AJayen_US
dc.contributor.authorLi, Huen_US
dc.contributor.authorZhang, Jinen_US
dc.contributor.authorPardee, Keithen_US
dc.contributor.authorGennert, Daviden_US
dc.contributor.authorTrombetta, John J.en_US
dc.contributor.authorFerrante, Thomas C.en_US
dc.contributor.authorRegev, Aviven_US
dc.contributor.authorDaley, George Q.en_US
dc.contributor.authorCollins, James J.en_US
dc.date.accessioned2015-07-13T18:47:43Z
dc.date.issued2014en_US
dc.identifier.citationKumar, R. M., P. Cahan, A. K. Shalek, R. Satija, A. DaleyKeyser, H. Li, J. Zhang, et al. 2014. “Deconstructing transcriptional heterogeneity in pluripotent stem cells.” Nature 516 (7529): 56-61. doi:10.1038/nature13920. http://dx.doi.org/10.1038/nature13920.en
dc.identifier.issn0028-0836en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17295753
dc.description.abstractSUMMARY Pluripotent stem cells (PSCs) are capable of dynamic interconversion between distinct substates, but the regulatory circuits specifying these states and enabling transitions between them are not well understood. We set out to characterize transcriptional heterogeneity in PSCs by single-cell expression profiling under different chemical and genetic perturbations. Signaling factors and developmental regulators show highly variable expression, with expression states for some variable genes heritable through multiple cell divisions. Expression variability and population heterogeneity can be influenced by perturbation of signaling pathways and chromatin regulators. Strikingly, either removal of mature miRNAs or pharmacologic blockage of signaling pathways drives PSCs into a low-noise ground state characterized by a reconfigured pluripotency network, enhanced self-renewal, and a distinct chromatin state, an effect mediated by opposing miRNA families acting on the c-myc / Lin28 / let-7 axis. These data illuminate the nature of transcriptional heterogeneity in PSCs.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/nature13920en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4256722/pdf/en
dash.licenseLAAen_US
dc.titleDeconstructing transcriptional heterogeneity in pluripotent stem cellsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNatureen
dash.depositing.authorCahan, Patricken_US
dc.date.available2015-07-13T18:47:43Z
dc.identifier.doi10.1038/nature13920*
dash.authorsorderedfalse
dash.contributor.affiliatedZhang, Jin
dash.contributor.affiliatedFerrante, Thomas
dash.contributor.affiliatedCahan, Patrick
dash.contributor.affiliatedDaley, George
dash.contributor.affiliatedCollins, James


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