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dc.contributor.authorKaya, Alaattinen_US
dc.contributor.authorLobanov, Alexei Ven_US
dc.contributor.authorGladyshev, Vadim Nen_US
dc.date.accessioned2015-07-13T18:47:48Z
dc.date.issued2014en_US
dc.identifier.citationKaya, Alaattin, Alexei V Lobanov, and Vadim N Gladyshev. 2014. “Evidence that mutation accumulation does not cause aging in Saccharomyces cerevisiae.” Aging Cell 14 (3): 366-371. doi:10.1111/acel.12290. http://dx.doi.org/10.1111/acel.12290.en
dc.identifier.issn1474-9718en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17295764
dc.description.abstractThe concept that mutations cause aging phenotypes could not be directly tested previously due to inability to identify age-related mutations in somatic cells and determine their impact on organismal aging. Here, we subjected Saccharomyces cerevisiae to multiple rounds of replicative aging and assessed de novo mutations in daughters of mothers of different age. Mutations did increase with age, but their low numbers, < 1 per lifespan, excluded their causal role in aging. Structural genome changes also had no role. A mutant lacking thiol peroxidases had the mutation rate well above that of wild-type cells, but this did not correspond to the aging pattern, as old wild-type cells with few or no mutations were dying, whereas young mutant cells with many more mutations continued dividing. In addition, wild-type cells lost mitochondrial DNA during aging, whereas shorter-lived mutant cells preserved it, excluding a causal role of mitochondrial mutations in aging. Thus, DNA mutations do not cause aging in yeast. These findings may apply to other damage types, suggesting a causal role of cumulative damage, as opposed to individual damage types, in organismal aging.en
dc.language.isoen_USen
dc.publisherBlackWell Publishing Ltden
dc.relation.isversionofdoi:10.1111/acel.12290en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4406665/pdf/en
dash.licenseLAAen_US
dc.subjectagingen
dc.subjectDNA damageen
dc.subjectlifespanen
dc.subjectmitochondriaen
dc.subjectmutationsen
dc.subjectthiol peroxidaseen
dc.subjectyeasten
dc.titleEvidence that mutation accumulation does not cause aging in Saccharomyces cerevisiaeen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalAging Cellen
dash.depositing.authorKaya, Alaattinen_US
dc.date.available2015-07-13T18:47:48Z
dc.identifier.doi10.1111/acel.12290*
dash.contributor.affiliatedKaya, Alaattin
dash.contributor.affiliatedGladyshev, Vadim
dash.contributor.affiliatedLobanov, Alexei


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