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dc.contributor.authorGao, Yanen_US
dc.contributor.authorFeng, Yongen_US
dc.contributor.authorShen, Jacson K.en_US
dc.contributor.authorLin, Minen_US
dc.contributor.authorChoy, Edwinen_US
dc.contributor.authorCote, Gregory M.en_US
dc.contributor.authorHarmon, David C.en_US
dc.contributor.authorMankin, Henry J.en_US
dc.contributor.authorHornicek, Francis J.en_US
dc.contributor.authorDuan, Zhenfengen_US
dc.date.accessioned2015-07-13T18:47:48Z
dc.date.issued2015en_US
dc.identifier.citationGao, Yan, Yong Feng, Jacson K. Shen, Min Lin, Edwin Choy, Gregory M. Cote, David C. Harmon, Henry J. Mankin, Francis J. Hornicek, and Zhenfeng Duan. 2015. “CD44 is a direct target of miR-199a-3p and contributes to aggressive progression in osteosarcoma.” Scientific Reports 5 (1): 11365. doi:10.1038/srep11365. http://dx.doi.org/10.1038/srep11365.en
dc.identifier.issn2045-2322en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17295765
dc.description.abstractOsteosarcoma is the most common primary bone malignancy in children and adolescents. Herein, we investigated the role of cluster of differentiation 44 (CD44), a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration in osteosarcoma. We constructed a human osteosarcoma tissue microarray with 114 patient tumor specimens, including tumor tissues from primary, metastatic, and recurrent stages, and determined the expression of CD44 by immunohistochemistry. Results showed that CD44 was overexpressed in metastatic and recurrent osteosarcoma as compared with primary tumors. Higher expression of CD44 was found in both patients with shorter survival and patients who exhibited unfavorable response to chemotherapy before surgical resection. Additionally, the 3′-untranslated region of CD44 mRNA was the direct target of microRNA-199a-3p (miR-199a-3p). Overexpression of miR-199a-3p significantly inhibited CD44 expression in osteosarcoma cells. miR-199a-3p is one of the most dramatically decreased miRs in osteosarcoma cells and tumor tissues as compared with normal osteoblast cells. Transfection of miR-199a-3p significantly increased the drug sensitivity through down-regulation of CD44 in osteosarcoma cells. Taken together, these results suggest that the CD44-miR-199a-3p axis plays an important role in the development of metastasis, recurrence, and drug resistance of osteosarcoma. Developing strategies to target CD44 may improve the clinical outcome of osteosarcoma.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/srep11365en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468826/pdf/en
dash.licenseLAAen_US
dc.titleCD44 is a direct target of miR-199a-3p and contributes to aggressive progression in osteosarcomaen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalScientific Reportsen
dash.depositing.authorGao, Yanen_US
dc.date.available2015-07-13T18:47:48Z
dc.identifier.doi10.1038/srep11365*
dash.contributor.affiliatedLin, Min
dash.contributor.affiliatedHarmon, David
dash.contributor.affiliatedHornicek, Francis
dash.contributor.affiliatedCote, Gregory
dash.contributor.affiliatedMankin, Henry
dash.contributor.affiliatedGao, Yan
dash.contributor.affiliatedChoy, Edwin


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