Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes

DSpace/Manakin Repository

Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes

Citable link to this page

 

 
Title: Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes
Author: Islam, Rakibul; Jackson, Catherine; Eidet, Jon R.; Messelt, Edward B.; Corraya, Rima Maria; Lyberg, Torstein; Griffith, May; Dartt, Darlene A.; Utheim, Tor P.

Note: Order does not necessarily reflect citation order of authors.

Citation: Islam, Rakibul, Catherine Jackson, Jon R. Eidet, Edward B. Messelt, Rima Maria Corraya, Torstein Lyberg, May Griffith, Darlene A. Dartt, and Tor P. Utheim. 2015. “Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes.” PLoS ONE 10 (6): e0128306. doi:10.1371/journal.pone.0128306. http://dx.doi.org/10.1371/journal.pone.0128306.
Full Text & Related Files:
Abstract: Purpose/Aims To assess the effect of storage temperature on the viability, phenotype, metabolism, and morphology of cultured human oral keratinocytes (HOK). Materials and Methods Cultured HOK cells were stored in HEPES- and sodium bicarbonate-buffered Minimum Essential Medium (MEM) at nine temperatures in approximately 4°C increments from 4°C to 37°C for seven days. Cells were characterized for viability by calcein fluorescence, phenotype retention by immunocytochemistry, metabolic parameters (pH, glucose, lactate, and O2) within the storage medium by blood gas analysis, and morphology by scanning electron microscopy and light microscopy. Results: Relative to the cultured, but non-stored control cells, a high percentage of viable cells were retained only in the 12°C and 16°C storage groups (85%±13% and 68%±10%, respectively). Expression of ABCG2, Bmi1, C/EBPδ, PCNA, cytokeratin 18, and caspase-3 were preserved after storage in the 5 groups between 4°C and 20°C, compared to the non-stored control. Glucose, pH and pO2 in the storage medium declined, whereas lactate increased with increasing storage temperature. Morphology was best preserved following storage of the three groups between 12°C, 16°C, and 20°C. Conclusion: We conclude that storage temperatures of 12°C and 16°C were optimal for maintenance of cell viability, phenotype, and morphology of cultured HOK. The storage method described in the present study may be applicable for other cell types and tissues; thus its significance may extend beyond HOK and the field of ophthalmology.
Published Version: doi:10.1371/journal.pone.0128306
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459984/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:17295777
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters