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dc.contributor.authorPerez, Julio Den_US
dc.contributor.authorRubinstein, Nimrod Den_US
dc.contributor.authorFernandez, Daniel Een_US
dc.contributor.authorSantoro, Stephen Wen_US
dc.contributor.authorNeedleman, Leigh Aen_US
dc.contributor.authorHo-Shing, Oliviaen_US
dc.contributor.authorChoi, John Jen_US
dc.contributor.authorZirlinger, Marielaen_US
dc.contributor.authorChen, Shau-Kwaunen_US
dc.contributor.authorLiu, Jun Sen_US
dc.contributor.authorDulac, Catherineen_US
dc.date.accessioned2015-08-03T13:59:11Z
dc.date.issued2015en_US
dc.identifier.citationPerez, J. D., N. D. Rubinstein, D. E. Fernandez, S. W. Santoro, L. A. Needleman, O. Ho-Shing, J. J. Choi, et al. 2015. “Quantitative and functional interrogation of parent-of-origin allelic expression biases in the brain.” eLife 4 (1): e07860. doi:10.7554/eLife.07860. http://dx.doi.org/10.7554/eLife.07860.en
dc.identifier.issn2050-084Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17820657
dc.description.abstractThe maternal and paternal genomes play different roles in mammalian brains as a result of genomic imprinting, an epigenetic regulation leading to differential expression of the parental alleles of some genes. Here we investigate genomic imprinting in the cerebellum using a newly developed Bayesian statistical model that provides unprecedented transcript-level resolution. We uncover 160 imprinted transcripts, including 41 novel and independently validated imprinted genes. Strikingly, many genes exhibit parentally biased—rather than monoallelic—expression, with different magnitudes according to age, organ, and brain region. Developmental changes in parental bias and overall gene expression are strongly correlated, suggesting combined roles in regulating gene dosage. Finally, brain-specific deletion of the paternal, but not maternal, allele of the paternally-biased Bcl-x, (Bcl2l1) results in loss of specific neuron types, supporting the functional significance of parental biases. These findings reveal the remarkable complexity of genomic imprinting, with important implications for understanding the normal and diseased brain. DOI: http://dx.doi.org/10.7554/eLife.07860.001en
dc.language.isoen_USen
dc.publishereLife Sciences Publications, Ltden
dc.relation.isversionofdoi:10.7554/eLife.07860en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512258/pdf/en
dash.licenseLAAen_US
dc.subjectgenomic imprintingen
dc.subjectmolecular neuroscienceen
dc.subjectcerebellumen
dc.subjectRNA-seqen
dc.subjectapoptosisen
dc.subjectBcl-xen
dc.subjectmouseen
dc.titleQuantitative and functional interrogation of parent-of-origin allelic expression biases in the brainen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journaleLifeen
dash.depositing.authorPerez, Julio Den_US
dc.date.available2015-08-03T13:59:11Z
dc.identifier.doi10.7554/eLife.07860*
dash.authorsorderedfalse
dash.identifier.orcid0000-0002-8769-9306en_US
dash.contributor.affiliatedHo-Shing, Olivia
dash.contributor.affiliatedNeedleman, Leigh
dash.contributor.affiliatedChoi, John J
dash.contributor.affiliatedRubinstein, Nimrod
dash.contributor.affiliatedPerez, Julio
dash.contributor.affiliatedLiu, Jun
dash.contributor.affiliatedDulac, Catherine
dc.identifier.orcid0000-0002-8769-9306


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