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dc.contributor.authorMcCall, Keisha C.en_US
dc.contributor.authorCheng, Su-Chunen_US
dc.contributor.authorHuang, Yingen_US
dc.contributor.authorKohl, Nancy E.en_US
dc.contributor.authorTupper, Tanyaen_US
dc.contributor.authorVan den Abbeele, Annick D.en_US
dc.contributor.authorZukotynski, Katherine A.en_US
dc.contributor.authorSweeney, Christopher J.en_US
dc.date.accessioned2015-08-03T14:01:57Z
dc.date.issued2015en_US
dc.identifier.citationMcCall, Keisha C., Su-Chun Cheng, Ying Huang, Nancy E. Kohl, Tanya Tupper, Annick D. Van den Abbeele, Katherine A. Zukotynski, and Christopher J. Sweeney. 2015. “[18F]-Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography of LAPC4-CR Castration-Resistant Prostate Cancer Xenograft Model in Soft Tissue Compartments1.” Translational Oncology 8 (3): 147-153. doi:10.1016/j.tranon.2015.03.004. http://dx.doi.org/10.1016/j.tranon.2015.03.004.en
dc.identifier.issn1936-5233en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17820818
dc.description.abstractPreclinical xenograft models have contributed to advancing our understanding of the molecular basis of prostate cancer and to the development of targeted therapy. However, traditional preclinical in vivo techniques using caliper measurements and survival analysis evaluate the macroscopic tumor behavior, whereas tissue sampling disrupts the microenvironment and cannot be used for longitudinal studies in the same animal. Herein, we present an in vivo study of [18F]-fluorodeoxyglucose (FDG) positron emission tomography (PET)/computed tomography (CT) designed to evaluate the metabolism within the microenvironment of LAPC4-CR, a unique murine model of castration-resistant prostate cancer. Mice bearing LAPC4-CR subcutaneous tumors were administered [18F]-FDG via intravenous injection. After a 60-minute distribution phase, the mice were imaged on a PET/CT scanner with submillimeter resolution; and the fused PET/CT images were analyzed to evaluate tumor size, location, and metabolism across the cohort of mice. The xenograft tumors showed [18F]-FDG uptake that was independent of tumor size and was significantly greater than uptake in skeletal muscle and liver in mice (Wilcoxon signed-rank P values of .0002 and .0002, respectively). [18F]-FDG metabolism of the LAPC4-CR tumors was 2.1 ± 0.8 ID/cm3*wt, with tumor to muscle ratio of 7.4 ± 4.7 and tumor to liver background ratio of 6.7 ± 2.3. Noninvasive molecular imaging techniques such as PET/CT can be used to probe the microenvironment of tumors in vivo. This study showed that [18F]-FDG-PET/CT could be used to image and assess glucose metabolism of LAPC4-CR xenografts in vivo. Further work can investigate the use of PET/CT to quantify the metabolic response of LAPC4-CR to novel agents and combination therapies using soft tissue and possibly bone compartment xenograft models.en
dc.language.isoen_USen
dc.publisherNeoplasia Pressen
dc.relation.isversionofdoi:10.1016/j.tranon.2015.03.004en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4487789/pdf/en
dash.licenseLAAen_US
dc.title[18F]-Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography of LAPC4-CR Castration-Resistant Prostate Cancer Xenograft Model in Soft Tissue Compartments1en
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalTranslational Oncologyen
dash.depositing.authorVan den Abbeele, Annick D.en_US
dc.date.available2015-08-03T14:01:57Z
dc.identifier.doi10.1016/j.tranon.2015.03.004*
dash.contributor.affiliatedVan Den Abbeele, Annick
dash.contributor.affiliatedSweeney, Christopher


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