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dc.contributor.authorWang, Boen_US
dc.contributor.authorLi, Dongpingen_US
dc.contributor.authorSidler, Corinneen_US
dc.contributor.authorRodriguez-Juarez, Rocioen_US
dc.contributor.authorSingh, Natashaen_US
dc.contributor.authorHeyns, Miekeen_US
dc.contributor.authorIlnytskyy, Yaroslaven_US
dc.contributor.authorBronson, Roderick T.en_US
dc.contributor.authorKovalchuk, Olgaen_US
dc.date.accessioned2015-08-03T14:02:01Z
dc.date.issued2015en_US
dc.identifier.citationWang, Bo, Dongping Li, Corinne Sidler, Rocio Rodriguez-Juarez, Natasha Singh, Mieke Heyns, Yaroslav Ilnytskyy, Roderick T. Bronson, and Olga Kovalchuk. 2015. “A suppressive role of ionizing radiation-responsive miR-29c in the development of liver carcinoma via targeting WIP1.” Oncotarget 6 (12): 9937-9950.en
dc.identifier.issn1949-2553en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17820825
dc.description.abstractHepatocellular carcinoma (HCC) is the third most common cause of cancer-related deaths worldwide, and it has been linked to radiation exposure. As a well-defined oncogene, wild-type p53-induced phosphatase 1 (WIP1) plays an inhibitory role in several tumor suppressor pathways, including p53. WIP1 is amplified and overexpressed in many malignancies, including HCC. However, the underlying mechanisms remain largely unknown. Here, we show that low-dose ionizing radiation (IR) induces miR-29c expression in female mouse liver, while inhibiting its expression in HepG2, a human hepatocellular carcinoma cell line which is used as a model system in this study. miR-29c expression is downregulated in human hepatocellular carcinoma cells, which is inversely correlated with WIP1 expression. miR-29c attenuates luciferase activity of a reporter harboring the 3′UTR binding motif of WIP1 mRNA. Ectopic expression of miR-29c significantly represses cell proliferation and induces apoptosis and G1 arrest in HepG2. In contrast, the knockdown of miR-29c greatly enhances HepG2 cell proliferation and suppresses apoptosis. The biological effects of miR-29c may be mediated by its target WIP1 which regulates p53 activity via dephosphorylation at Ser-15. Finally, fluorescence in situ hybridization (FISH) and immunohistochemical analyses indicate that miR-29c is downregulated in 50.6% of liver carcinoma tissues examined, whereas WIP1 is upregulated in 45.4% of these tissues. The expression of miR-29c inversely correlates with that of WIP1 in HCC. Our results suggest that the IR-responsive miR-29c may function as a tumor suppressor that plays a crucial role in the development of liver carcinoma via targeting WIP1, therefore possibly representing a target molecule for therapeutic intervention for this disease.en
dc.language.isoen_USen
dc.publisherImpact Journals LLCen
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496408/pdf/en
dash.licenseLAAen_US
dc.subjectionizing radiationen
dc.subjectmiR-29cen
dc.subjecthepatocellular carcinomaen
dc.subjectWIP1en
dc.titleA suppressive role of ionizing radiation-responsive miR-29c in the development of liver carcinoma via targeting WIP1en
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalOncotargeten
dash.depositing.authorBronson, Roderick T.en_US
dc.date.available2015-08-03T14:02:01Z
dash.contributor.affiliatedBronson, Roderick


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