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dc.contributor.authorJung, Yookyungen_US
dc.contributor.authorTam, Joshuaen_US
dc.contributor.authorJalian, H. Rayen_US
dc.contributor.authorAnderson, R. Roxen_US
dc.contributor.authorEvans, Conor L.en_US
dc.date.accessioned2015-08-03T14:02:06Z
dc.date.issued2014en_US
dc.identifier.citationJung, Yookyung, Joshua Tam, H. Ray Jalian, R. Rox Anderson, and Conor L. Evans. 2014. “Longitudinal, 3D in vivo imaging of sebaceous glands by coherent anti-Stokes Raman scattering microscopy –normal function and response to cryotherapy.” The Journal of investigative dermatology 135 (1): 39-44. doi:10.1038/jid.2014.293. http://dx.doi.org/10.1038/jid.2014.293.en
dc.identifier.issn0022-202Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17820832
dc.description.abstractSebaceous glands perform complex functions, and are centrally involved in the pathogenesis of acne vulgaris. Current techniques for studying sebaceous glands are mostly static in nature, whereas the gland’s main function – excretion of sebum via the holocrine mechanism – can only be evaluated over time. We present a longitudinal, real-time alternative – the in vivo, label-free imaging of sebaceous glands using Coherent Anti-Stokes Raman Scattering (CARS) microscopy, which is used to selectively visualize lipids. In mouse ears, CARS microscopy revealed dynamic changes in sebaceous glands during the holocrine secretion process, as well as in response to damage to the glands caused by cooling. Detailed gland structure, plus the active migration of individual sebocytes and cohorts of sebocytes were measured. Cooling produced characteristic changes in sebocyte structure and migration. This study demonstrates that CARS microscopy is a promising tool for studying the sebaceous gland and its associated disorders in three-dimensions in vivo.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/jid.2014.293en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4268001/pdf/en
dash.licenseLAAen_US
dc.titleLongitudinal, 3D in vivo imaging of sebaceous glands by coherent anti-Stokes Raman scattering microscopy –normal function and response to cryotherapyen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalThe Journal of investigative dermatologyen
dash.depositing.authorJung, Yookyungen_US
dc.date.available2015-08-03T14:02:06Z
dc.identifier.doi10.1038/jid.2014.293*
dash.contributor.affiliatedTam, Joshua
dash.contributor.affiliatedEvans, Conor
dash.contributor.affiliatedJung, Yookyung


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