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dc.contributor.authorShiwaku, Yukarien_US
dc.contributor.authorNeff, Lynnen_US
dc.contributor.authorNagano, Kenichien_US
dc.contributor.authorTakeyama, Ken-Ichien_US
dc.contributor.authorde Bruijn, Joosten_US
dc.contributor.authorDard, Michelen_US
dc.contributor.authorGori, Francescaen_US
dc.contributor.authorBaron, Rolanden_US
dc.date.accessioned2015-08-03T14:02:15Z
dc.date.issued2015en_US
dc.identifier.citationShiwaku, Yukari, Lynn Neff, Kenichi Nagano, Ken-Ichi Takeyama, Joost de Bruijn, Michel Dard, Francesca Gori, and Roland Baron. 2015. “The Crosstalk between Osteoclasts and Osteoblasts Is Dependent upon the Composition and Structure of Biphasic Calcium Phosphates.” PLoS ONE 10 (7): e0132903. doi:10.1371/journal.pone.0132903. http://dx.doi.org/10.1371/journal.pone.0132903.en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17820859
dc.description.abstractBiphasic calcium phosphates (BCPs), consisting of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), exhibit good biocompatibility and osteoconductivity, maintaining a balance between resorption of the biomaterial and formation of new bone. We tested whether the chemical composition and/or the microstructure of BCPs affect osteoclasts (OCs) differentiation and/or their ability to crosstalk with osteoblasts (OBs). To this aim, OCs were cultured on BCPs with HA content of 5, 20 or 60% and their differentiation and activity were assessed. We found that OC differentiation is partially impaired by increased HA content, but not by the presence of micropores within BCP scaffolds, as indicated by TRAP staining and gene profile expression. We then investigated whether the biomaterial-induced changes in OC differentiation also affect their ability to crosstalk with OBs and regulate OB function. We found that BCPs with low percentage of HA favored the expression of positive coupling factors, including sphingosine-kinase 1 (SPHK1) and collagen triple helix repeat containing 1 (Cthrc1). In turn, the increase of these secreted coupling factors promotes OB differentiation and function. All together our studies suggest that the chemical composition of biomaterials affects not only the differentiation and activity of OCs but also their potential to locally regulate bone formation.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.relation.isversionofdoi:10.1371/journal.pone.0132903en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507990/pdf/en
dash.licenseLAAen_US
dc.titleThe Crosstalk between Osteoclasts and Osteoblasts Is Dependent upon the Composition and Structure of Biphasic Calcium Phosphatesen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalPLoS ONEen
dash.depositing.authorNagano, Kenichien_US
dc.date.available2015-08-03T14:02:15Z
dc.identifier.doi10.1371/journal.pone.0132903*
dash.contributor.affiliatedGori, Francesca
dash.contributor.affiliatedNagano, Kenichi
dash.contributor.affiliatedBaron, Roland


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