Show simple item record

dc.contributor.authorDuquette, Marken_US
dc.contributor.authorNadler, Monicaen_US
dc.contributor.authorOkuhara, Dayneen_US
dc.contributor.authorThompson, Jillen_US
dc.contributor.authorShuttleworth, Trevoren_US
dc.contributor.authorLawler, Jacken_US
dc.date.accessioned2015-08-03T14:02:29Z
dc.date.issued2014en_US
dc.identifier.citationDuquette, Mark, Monica Nadler, Dayne Okuhara, Jill Thompson, Trevor Shuttleworth, and Jack Lawler. 2014. “Members of the thrombospondin gene family bind stromal interaction molecule 1 and regulate calcium channel activity.” Matrix biology : journal of the International Society for Matrix Biology 37 (1): 15-24. doi:10.1016/j.matbio.2014.05.004. http://dx.doi.org/10.1016/j.matbio.2014.05.004.en
dc.identifier.issn0945-053Xen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17820894
dc.description.abstractThe thrombospondins (TSPs) are a family of matricellular proteins that regulate cellular phenotype through interactions with a myriad of other proteins and proteoglycans. We have identified a novel interaction of the members of the TSP gene family with stromal interaction molecule 1 (STIM1). This association is robust since it is preserved in Triton X-100, can be detected with multiple anti-TSP-1 and anti-STIM1 antibodies, and is detected in a wide range of cell types. We have also found that STIM1 co-immunoprecipitates with TSP-4 and cartilage oligomeric matrix protein (COMP), and that a recombinant version of the N-terminal domain of STIM1 binds to the signature domain of TSP-1 and COMP. The association of the TSPs with STIM1 is observed in both the presence and absence of calcium indicating that the calcium-dependent conformation of the signature domain of TSPs is not required for binding. Thus, this interaction could occur in the ER under conditions of normal or low calcium concentration. Furthermore, we observed that the expression of COMP in HEK 293 cells decreases STIM1-mediated calcium release activated calcium (CRAC) channel currents and increases arachidonic acid calcium (ARC) channel currents. These data indicate that the TSPs regulate STIM1 function and participate in the reciprocal regulation of two channels that mediate calcium entry into the cell.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1016/j.matbio.2014.05.004en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4502920/pdf/en
dash.licenseLAAen_US
dc.subjectThrombospondinen
dc.subjectStromal interaction molecule 1en
dc.subjectCalcium signalingen
dc.subjectArachidonate-regulated calcium channelen
dc.titleMembers of the thrombospondin gene family bind stromal interaction molecule 1 and regulate calcium channel activityen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalMatrix biology : journal of the International Society for Matrix Biologyen
dash.depositing.authorNadler, Monicaen_US
dc.date.available2015-08-03T14:02:29Z
dc.identifier.doi10.1016/j.matbio.2014.05.004*
dash.contributor.affiliatedLawler, Jack
dash.contributor.affiliatedNadler, Monica


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record