Temporal regulation of HIF-1 and NF-κB in hypoxic hepatocarcinoma cells

View/ Open
Author
Jiang, Yuan
Zhu, Ying
Wang, Xinxin
Gong, Juan
Hu, Chunyan
Guo, Bo
Zhu, Bo
Li, Yongsheng
Note: Order does not necessarily reflect citation order of authors.
Metadata
Show full item recordCitation
Jiang, Yuan, Ying Zhu, Xinxin Wang, Juan Gong, Chunyan Hu, Bo Guo, Bo Zhu, and Yongsheng Li. 2015. “Temporal regulation of HIF-1 and NF-κB in hypoxic hepatocarcinoma cells.” Oncotarget 6 (11): 9409-9419.Abstract
Regulations between NF-κB and HIF-1 have not been adequately addressed in previous research. Here, we report that hypoxia increased NF-κB in hepatocellular carcinoma cells. The HIF-1 protein level was rapidly induced by protein stabilization (by 2 hours) and then moderately decreased, whereas mRNA levels were reciprocally increased. We also found that NF-κB p50 and p65 (RelA), but not c-Rel, bound the HIF-1a promoter, thus increasing its transcription. In contrast, miR-199a-5p and miR-93, c-Rel downstream targets, decreased HIF-1α at both the mRNA and protein levels. Dicer1, a key enzyme in miRNA biogenesis, was decreased by acute hypoxia but was later increased by HIF-1, rather than by the above-mentioned NF-κB subunits. Thus, NF-κB both positively and negatively fine-tuned HIF-1 in hypoxic hepatocarcinoma cells.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496226/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:17820915
Collections
- HMS Scholarly Articles [17875]
Contact administrator regarding this item (to report mistakes or request changes)