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dc.contributor.authorJiang, Yuanen_US
dc.contributor.authorZhu, Yingen_US
dc.contributor.authorWang, Xinxinen_US
dc.contributor.authorGong, Juanen_US
dc.contributor.authorHu, Chunyanen_US
dc.contributor.authorGuo, Boen_US
dc.contributor.authorZhu, Boen_US
dc.contributor.authorLi, Yongshengen_US
dc.date.accessioned2015-08-03T14:02:35Z
dc.date.issued2015en_US
dc.identifier.citationJiang, Yuan, Ying Zhu, Xinxin Wang, Juan Gong, Chunyan Hu, Bo Guo, Bo Zhu, and Yongsheng Li. 2015. “Temporal regulation of HIF-1 and NF-κB in hypoxic hepatocarcinoma cells.” Oncotarget 6 (11): 9409-9419.en
dc.identifier.issn1949-2553en
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17820915
dc.description.abstractRegulations between NF-κB and HIF-1 have not been adequately addressed in previous research. Here, we report that hypoxia increased NF-κB in hepatocellular carcinoma cells. The HIF-1 protein level was rapidly induced by protein stabilization (by 2 hours) and then moderately decreased, whereas mRNA levels were reciprocally increased. We also found that NF-κB p50 and p65 (RelA), but not c-Rel, bound the HIF-1a promoter, thus increasing its transcription. In contrast, miR-199a-5p and miR-93, c-Rel downstream targets, decreased HIF-1α at both the mRNA and protein levels. Dicer1, a key enzyme in miRNA biogenesis, was decreased by acute hypoxia but was later increased by HIF-1, rather than by the above-mentioned NF-κB subunits. Thus, NF-κB both positively and negatively fine-tuned HIF-1 in hypoxic hepatocarcinoma cells.en
dc.language.isoen_USen
dc.publisherImpact Journals LLCen
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496226/pdf/en
dash.licenseLAAen_US
dc.subjecthypoxiaen
dc.subjectNF-κBen
dc.subjectHIF-1en
dc.subjectmiRNAen
dc.titleTemporal regulation of HIF-1 and NF-κB in hypoxic hepatocarcinoma cellsen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalOncotargeten
dc.date.available2015-08-03T14:02:35Z
dash.authorsorderedfalse


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