Primary microRNA processing is functionally coupled to RNAP II transcription in vitro
Citation
Yin, Shanye, Yong Yu, and Robin Reed. 2015. “Primary microRNA processing is functionally coupled to RNAP II transcription in vitro.” Scientific Reports 5 (1): 11992. doi:10.1038/srep11992. http://dx.doi.org/10.1038/srep11992.Abstract
Previous studies in vivo reported that processing of primary microRNA (pri-miRNA) is coupled to transcription by RNA polymerase II (RNAP II) and can occur co-transcriptionally. Here we have established a robust in vivo system in which pri-miRNA is transcribed by RNAP II and processed to pre-miRNA in HeLa cell nuclear extracts. We show that both the kinetics and efficiency of pri-miRNA processing are dramatically enhanced in this system compared to that of the corresponding naked pri-miRNA. Moreover, this enhancement is general as it occurs with multiple pri-miRNAs. We also show that nascent pri-miRNA is efficiently processed before it is released from the DNA template. Together, our work directly demonstrates that transcription and pri-miRNA processing are functionally coupled and establishes the first in vivo model systems for this functional coupling and for co-transcriptional processing.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493704/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:17820941
Collections
- HMS Scholarly Articles [17298]
Contact administrator regarding this item (to report mistakes or request changes)