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dc.contributor.authorAgarwal, Ashutosh
dc.contributor.authorGoss, Josue Adrian
dc.contributor.authorCho, Alexander
dc.contributor.authorMcCain, Megan Laura
dc.contributor.authorParker, Kevin Kit
dc.date.accessioned2015-08-10T17:09:30Z
dc.date.issued2013
dc.identifier.citationAgarwal, Ashutosh, Josue Adrian Goss, Alexander Cho, Megan Laura McCain, and Kevin Kit Parker. 2013. “Microfluidic Heart on a Chip for Higher Throughput Pharmacological Studies.” Lab Chip 13 (18): 3599. doi:10.1039/c3lc50350j. http://dx.doi.org/10.1039/c3lc50350j.en_US
dc.identifier.issn1473-0197en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:17985229
dc.description.abstractWe present the design of a higher throughput “heart on a chip” which utilizes a semi-automated fabrication technique to process sub millimeter sized thin film cantilevers of soft elastomers. Anisotropic cardiac microtissues which recapitulate the laminar architecture of the heart ventricle are engineered on these cantilevers. Deflection of these cantilevers, termed Muscular Thin Films (MTFs), during muscle contraction allows calculation of diastolic and systolic stresses generated by the engineered tissues. We also present the design of a reusable one channel fluidic microdevice completely built out of autoclavable materials which incorporates various features required for an optical cardiac contractility assay: metallic base which fits on a heating element for temperature control, transparent top for recording cantilever deformation and embedded electrodes for electrical field stimulation of the tissue. We employ the microdevice to test the positive inotropic effect of isoproterenol on cardiac contractility at dosages ranging from 1 nM to 100 μM. The higher throughput fluidic heart on a chip has applications in testing of cardiac tissues built from rare or expensive cell sources and for integration with other organ mimics. These advances will help alleviate translational barriers for commercial adoption of these technologies by improving the throughput and reproducibility of readout, standardization of the platform and scalability of manufacture.en_US
dc.description.sponsorshipEngineering and Applied Sciencesen_US
dc.language.isoen_USen_US
dc.publisherRoyal Society of Chemistry (RSC)en_US
dc.relation.isversionof10.1039/c3lc50350jen_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3786400/en_US
dash.licenseOAP
dc.titleMicrofluidic heart on a chip for higher throughput pharmacological studiesen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalLab on a Chipen_US
dash.depositing.authorParker, Kevin Kit
dc.date.available2015-08-10T17:09:30Z
dc.identifier.doi10.1039/c3lc50350j*
workflow.legacycommentsFAR 2013en_US
dash.contributor.affiliatedParker, Kevin


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